2016年9月
Musashi mediates translational repression of the Drosophila hypoxia inducible factor
NUCLEIC ACIDS RESEARCH
- 巻
- 44
- 号
- 16
- 開始ページ
- 7555
- 終了ページ
- 7567
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1093/nar/gkw372
- 出版者・発行元
- OXFORD UNIV PRESS
Adaptation to hypoxia depends on a conserved alpha/beta heterodimeric transcription factor called Hypoxia Inducible Factor (HIF), whose alpha-subunit is regulated by oxygen through different concurrent mechanisms. In this study, we have identified the RNA binding protein dMusashi, as a negative regulator of the fly HIF homologue Sima. Genetic interaction assays suggested that dMusashi participates of the HIF pathway, and molecular studies carried out in Drosophila cell cultures showed that dMusashi recognizes a Musashi Binding Element in the 3' UTR of the HIF alpha transcript, thereby mediating its translational repression in normoxia. In hypoxic conditions dMusashi is downregulated, lifting HIF alpha repression and contributing to trigger HIF-dependent gene expression. Analysis performed in mouse brains revealed that murine Msi1 protein physically interacts with HIF-1 alpha transcript, suggesting that the regulation of HIF by Msi might be conserved in mammalian systems. Thus, Musashi is a novel regulator of HIF that inhibits responses to hypoxia specifically when oxygen is available.
- リンク情報
- ID情報
-
- DOI : 10.1093/nar/gkw372
- ISSN : 0305-1048
- eISSN : 1362-4962
- Web of Science ID : WOS:000384687000009