論文

査読有り
2016年9月

Musashi mediates translational repression of the Drosophila hypoxia inducible factor

NUCLEIC ACIDS RESEARCH
  • Agustina P. Bertolin
  • Maximiliano J. Katz
  • Masato Yano
  • Berta Pozzi
  • Julieta M. Acevedo
  • Dalmiro Blanco-Obregon
  • Lautaro Gandara
  • Eleonora Sorianello
  • Hiroshi Kanda
  • Hideyuki Okano
  • Anabella Srebrow
  • Pablo Wappner
  • 全て表示

44
16
開始ページ
7555
終了ページ
7567
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1093/nar/gkw372
出版者・発行元
OXFORD UNIV PRESS

Adaptation to hypoxia depends on a conserved alpha/beta heterodimeric transcription factor called Hypoxia Inducible Factor (HIF), whose alpha-subunit is regulated by oxygen through different concurrent mechanisms. In this study, we have identified the RNA binding protein dMusashi, as a negative regulator of the fly HIF homologue Sima. Genetic interaction assays suggested that dMusashi participates of the HIF pathway, and molecular studies carried out in Drosophila cell cultures showed that dMusashi recognizes a Musashi Binding Element in the 3' UTR of the HIF alpha transcript, thereby mediating its translational repression in normoxia. In hypoxic conditions dMusashi is downregulated, lifting HIF alpha repression and contributing to trigger HIF-dependent gene expression. Analysis performed in mouse brains revealed that murine Msi1 protein physically interacts with HIF-1 alpha transcript, suggesting that the regulation of HIF by Msi might be conserved in mammalian systems. Thus, Musashi is a novel regulator of HIF that inhibits responses to hypoxia specifically when oxygen is available.

リンク情報
DOI
https://doi.org/10.1093/nar/gkw372
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000384687000009&DestApp=WOS_CPL
ID情報
  • DOI : 10.1093/nar/gkw372
  • ISSN : 0305-1048
  • eISSN : 1362-4962
  • Web of Science ID : WOS:000384687000009

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