Indoleamine 2,3-dioxygenase1 (IDO1) is the rate-limiting enzyme in the kynurenine pathway that converts l-tryptophan to l-kynurenine. Encephalomyocarditis virus (EMCV) can cause acute myocarditis in various animals including mice. Previously, IDO1...
Keiko Abe, Keiko Abe, Phillip Evans, Elizabeth J. Austin, Yasuyuki Suzuki, Kazuhiko Fujisaki, Masayuki Niwa, Muneyoshi Aomatsu
BMC Medical Education 13 82 Jun 2013 [Refereed]
Background: There has been considerable interest in Emotional Intelligence (EI) in undergraduate medical education, with respect to student selection and admissions, health and well-being and academic performance. EI is a significant component of ...
Hypothermia: Prevention, Recognition and Treatment 137-146 Dec 2012
We describe hypothermia and hyperthermia mechanisms that affect delayed neuronal death following transient forebrain ischemia. Intra-ischemic, but not post-ischemic, hypothermia prevents delayed neuronal death and nuclear DNA fragmentation in hipp...
αB-crystallin, a low-molecular-weight heat shock protein (HSP), has binding sites on platelets. However, the exact role of αB-crystallin is not clarified. In this study, we investigated the effect of αB-crystallin on platelet granule secretion. αB...
Treatment of PBMCs with TNF-α decreased the levels of heat shock protein (HSP) 27, but had little effect on the level of HSP70. Parallel to the decrease of HSP27, TNF-α increased the level of HSP27 in the incubation medium of the cells. The decrea...
The actions of tumor necrosis factor-α (TNF-α) produced by resident brain cells and bone marrow-derived cells in brain following a transient global ischemia were evaluated. In wild-type mice (C57BI/6J) following 20 min ischemia with bilateral comm...
H. Tokuda, H. Tokuda, M. Niwa, A. Ishisaki, K. Nakajima, H. Ito, K. Kato, O. Kozawa
Prostaglandins Leukotrienes and Essential Fatty Acids 70 441-447 May 2004
We have reported that prostaglandin F2α (PGF 2α) activates p44/p42 mitogen-activated protein (MAP) kinase in osteoblast-like MC3T3-E1 cells, and that p44/p42 MAP kinase plays a role in the PGF2α-induced heat shock protein 27 (HSP27). In the presen...
H. Tokuda, H. Tokuda, M. Niwa, H. Ito, Y. Oiso, K. Kato, O. Kozawa
European Journal of Endocrinology 149 239-245 Sep 2003
Objective: We have reported that endothelin-1 (ET-1) activates p38 mitogen-activated protein (MAP) kinase through protein kinase C in osteoblast-like MC3T3-E1 cells, and that p38 MAP kinase plays a role in the ET-1-induced heat shock protein 27 (H...
American Journal of Physiology - Heart and Circulatory Physiology 283(3) H941-8 Sep 2002
We investigated the effects of thrombin on the induction of heat shock proteins (HSP) 70 and 27, and the mechanism behind the induction in aortic smooth muscle A10 cells. Thrombin increased the level of HSP27 but had little effect on the level of ...
Biochimica et Biophysica Acta - Molecular Cell Research 1589 15-30 Feb 2002
We investigated whether transforming growth factor-β (TGF-β) stimulates the induction of heat shock protein (HSP) 27 and HSP70 in osteoblast-like MC3T3-E1 cells and the mechanism underlying the induction. TGF-β increased the level of HSP27 but had...
H. Tokuda, O. Kozawa, M. Niwa, H. Matsuno, K. Kato, T. Uematsu
Journal of Endocrinology 172 271-281 Jan 2002
We investigated the effect of prostaglandin E2 (PGE2) on the induction of heat shock protein 27 (HSP27) and HSP70, and the mechanism behind the induction in osteoblast-like MC3T3-E1 cells. PGE2 time-dependently increased the level of HSP27 without...
A preliminary trial of an internet PBL tutorial using mailing list.
European Journal of Pharmacology 433(1) 17-27 Dec 2001
Tissue neutrophils, human salivary neutrophils donated from healthy subjects and synovial fluid neutrophils collected from patients with rheumatoid arthritis were compared with circulating blood neutrophils. Concomitant treatment of circulating bl...
Biological and Pharmaceutical Bulletin 24 1335-1337 Nov 2001
A variety of aromatic trifluoromethyl ketone derivatives has been studied as inhibitors of apoptosis in cerebellar granule neurons (CGNs). Among them, α-trifluoromethyl diketone (2) and benzyl trifluoromethyl ketone (11) were found to be apoptosis...
O. Kozawa, H. Matsuno, M. Niwa, D. Hatakeyama, K. Kato, T. Uematsu
Cell Stress and Chaperones 6 21-28 Jan 2001
It has recently been reported that αB-crystallin, a low-molecular-weight heat shock protein, may be released from cells by mechanical stretch. We investigated a physiological role of αB-crystallin in platelet function. αB-crystallin inhibited plat...
H. Matsuno, O. Kozawa, M. Niwa, S. Ueshima, O. Matsuo, D. Collen, T. Uematsu
Fibrinolysis and Proteolysis 14 13 Dec 2000
The role of fibrinolytic system components in thrombus formation and removal in vivo was studied in groups of six mice deficient in urokinasetype plasminogen activator (u-PA), tissue type plasminogen activator (t-PA) or plasminogen activator inhib...
European Journal of Pharmacology 407(3) 211-219 Nov 2000
The objective of this study was to elucidate the role of the nuclear factor-κB (NF-κB) pathway in tumor necrosis factor-α (TNF-α)-induced neutrophil apoptosis. A single treatment with TNF-α produced significant caspase-3 activiation in a time- and...
Journal of Cellular Biochemistry 75(4) 610-619 Dec 1999 [Refereed]
We previously reported that prostaglandin F(2α) (PGF(2α)) activates both phosphoinositide-hydrolyzing phospholipase C and phosphatidylcholine- hydrolyzing phospholipase D in osteoblast-like MC3T3-E1 cells and then induces the activation of protein...
We investigated the mechanism underlying the priming effect of TNF-α on fMLP-stimulated superoxide production in human neutrophils. TNF-α enhanced fMLP-stimulated superoxide production in a concentration-dependent manner. TNF-α also induced sphing...
We have shown that Hsp20, one of small molecular weight heat shock protein, which is present at a high concentration both in vascular smooth muscle cells and in circulating blood in patient with vascular disease, strongly inhibits platelet aggrega...
European Journal of Pharmacology 371(1) 59-67 Apr 1999 [Refereed]
Treatment of neutrophils with tumor necrosis factor-α (TNF-α) in the presence of cycloheximide induced apoptosis within 3 h, as evaluated by the occurrence of morphological nuclear changes characteristic of apoptosis. Pretreatment of neutrophils w...
It has been reported that delayed neuronal death (DND) in the hippocampus following transient forebrain ischemia is associated with internucleosomal DNA fragmentation, indicating apoptosis. This suggests that the process of DND is energy dependent...
Experimental Cell Research 246(2) 327-337 Feb 1999 [Refereed]
In the present study, we examined the effect of vasopressin on the induction of the low-molecular-weight heat shock proteins heat shock protein 27 (HSP27) and αB-crystallin in an aortic smooth muscle cell line, A10 cells. Vasopressin induced a tim...
Background: The mechanisms underlying the vascular effects of propofol are not fully understood. Vasopressin, a potent vasoactive peptide, stimulates the arachidonate cascade and the synthesis of prostacyclin (PGI2; the main metabolite of the casc...
Transport of fragmented DNA in apical dendrites of the CA1 pyramidal neurons of gerbil hippocampus is observed in the apoptotic process following transient fore ischemia. The time-course of specific DNA fragmentation was examined after the ischemi...
H. Matsuno, T. Kaida, M. Niwa, O. Kozawa, T. Uematsu
Fibrinolysis 10 53 Dec 1996
The long-lasting success of thrombolytic therapy is hampered by acute reocclusion due to thrombosis and by late restenosis due to smooth muscle cell migration and proliferation. Platelets play an importent role in stenosis and potent antiplatelet ...
M. Hattori, M. Nozaki, H. Shimonaka, M. Niwa, K. Kohno, S. Ohta, K. Tsurumi, S. Dohi
Anesthesia and Resuscitation 30 257-259 Dec 1994
Concerning about local anesthetics, the relationship between inhibitory effects on the superoxide anion (O2-) generation in human neutrophils and lipid solubility were studied. The O2- specific chemiluminescence development using a cypridina lucif...
Reportedly, nonsteroidal anti-inflammatory drugs (NSAIDs) especially the propionic acid derivatives are liable to cause convulsions when given to patients together with various new quinolone antimicrobials (NQs). Zaltoprofen, a novel NSAID, is a p...
Sparfloxacin (SPFX), a novel quinolone antibacterial (NQ) was compared with other NQs such as norfloxacin (NFLX), ofloxacin (OFLX), enoxacin (ENX), ciprofloxacin (CPFX), lomefloxacin (LFLX) and tosufloxacin-tosilate (TFLX) on the central nervous s...
T. Iwai, M. Niwa, S. Ohta, M. Nakashima, K. Tsurumi, M. Nozaki
New leads in opioid research: proceedings of the International Narcotics Research Conference. ICS914 362-363
Characterization of opioid receptors in cerebral ischemia was studied in Mongolian gerbils. The maximum binding capacities and dissociation constants for κ-type ligands were lowered by cerebral ischemia and were restored by 7 days reperfusion. Fro...
DNA fragmentation in gerbil hippocampus after transient forebrain ischemia: Its time course and effect of hypothermia
New leads in opioid research: proceedings of the International Narcotics Research Conference. ICS914 360-361
Mongolian gerbil cerebellum possessed a binding capacity for 3H-DAGO (μ agonist) but not for 3H-DPDPE (δ-agonist) or 3H-U-69593 (κ-agonist). The 3H-DAGO binding to the cerebellum was saturable, and characterized by high affinity (K(d) = 1.4 nM) an...
ed ER Giffard, Nova Science Publish, New York 2015
日本の医学教育の挑戦
鈴木康之、藤崎和彦、丹羽雅之編
篠原出版新社、東京 2012
Faculty developmentの企画・運営．日本の医学教育の挑戦
丹羽雅之
篠原出版新社、東京 2012
Hypothermia and Hyperthermia affect neuronal degeneration, delayed neuronal death and microglial activation following transient forebrain ischemi. In. Hypothermia: Prevention, Recognition and Treatment
Niwa M, Nakashima M, Satoh K, Takamatsu M, Kobayashi K, Hatano Y and Hara A (Part:Joint Work)
ed Delgado JIV and Garza VGF, Nova Science Publish 2012
Human embryonic stem cells transplanted into mouse retina induces neural differentiation. In Tumors of the central nervous system, In: Stem Cells and Cancer Stem Cells, Volume 2, Part 4
Hara A, Aoki H, Takamatu M, Hatano Y, Tomita H, Kuno K, Niwa M, Takahiro Kunisada (Part:Joint Work)
The ultimate goal of this study is treatment of retinal degeneration by regeneration. As a pre-stage, we constructed suitable regeneration condition using retinal degeneration model treating with CoCl2or NMDA. The results indicate hypoxia conditio...
NMDA receptors appear to be involved in activation of nociceptive neurons. The hyperexcitability of dorsal horm nociceptive neurons produced by formalin or inflammatory agents is blocked by NMDA antagonists. But it is not clear how nociceptive act...
To investigate the role of tissue plasminogen activator (tPA) in retina, we compared neuronal damage in the retina in tPA deficient, tPA(-/-), mice and their wild type. Retinal damage was induced by intravitreal injection of excitotoxins, N-methyl...
