Purpose: To report Stickler syndrome in a pair of female siblings. Cases: A 9-year-old girl was referred to us for rhegmatogenous retinal detachment in the right eye. She had moderate myopia and liquefied vitreous. She had also micrognathia and saddle nose, leading to the diagnosis of Stickler syndrome. Retinal detachment persisted after scleral buckling. The retina became reattached after 25-gauge microincision vitreous surgery 11 days later. Her sister, aged 7 years, had been diagnosed with Pierre-Robin syndrome due to micrognathia, cleft palate, and saddle nose. She showed myopia of about - 9 diopters with liquefied vitreous in both eyes. Their mother had received surgery for retinal detachment at the age of 17 years. Conclusion: These cases illustrate that Stickler syndrome may be an underlying factor for juvenile retinal detachment, for which microincision vitreous surgery may be effective.