2018年
Generation of Specific Aptamers.
Methods in molecular biology (Clifton, N.J.)
- ,
- ,
- 巻
- 1868
- 号
- 開始ページ
- 113
- 終了ページ
- 121
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1007/978-1-4939-8802-0_11
Nucleic acid aptamers are therapeutic agents consisting of short single-strand DNA or RNA oligonucleotides, which have the ability to bind to target therapeutic molecules with high affinity and specificity, and have been developed as potent drugs for the treatment of rheumatoid arthritis. Aptamers have unique and advantageous features over antibodies, such as superior affinity with nano- or pico-molar dissociation constants, and ease of chemical synthesis, modification, and inactivation by designing antisense sequences. In this chapter, using a DNA-oligonucleotide pool, the technology of proteoliposome-systematic evolution of ligands by exponential enrichment (SELEX) is introduced. By using this technique, potential therapeutic agents with high affinity and specificity could be obtained.
- リンク情報
- ID情報
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- DOI : 10.1007/978-1-4939-8802-0_11
- PubMed ID : 30244458