論文

査読有り 国際誌
2017年11月28日

A Highly Sensitive FRET Biosensor for AMPK Exhibits Heterogeneous AMPK Responses among Cells and Organs.

Cell reports
  • Yumi Konagaya
  • Kenta Terai
  • Yusuke Hirao
  • Kanako Takakura
  • Masamichi Imajo
  • Yuji Kamioka
  • Norio Sasaoka
  • Akira Kakizuka
  • Kenta Sumiyama
  • Tomoichiro Asano
  • Michiyuki Matsuda
  • 全て表示

21
9
開始ページ
2628
終了ページ
2638
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.celrep.2017.10.113
出版者・発行元
CELL PRESS

AMP-activated protein kinase (AMPK), a master regulator of cellular metabolism, is a potential target for type 2 diabetes. Although extensive in vitro studies have revealed the complex regulation of AMPK, much remains unknown about the regulation in vivo. We therefore developed transgenic mice expressing a highly sensitive fluorescence resonance energy transfer (FRET)-based biosensor for AMPK, called AMPKAR-EV. AMPKAR-EV allowed us to readily examine the role of LKB1, a canonical stimulator of AMPK, in drug-induced activation and inactivation of AMPK in vitro. In transgenic mice expressing AMPKAR-EV, the AMP analog AICAR activated AMPK in muscle. In contrast, the antidiabetic drug metformin activated AMPK in liver, highlighting the organ-specific action of AMPK stimulators. Moreover, we found that AMPK was activated primarily in fast-twitch muscle fibers after tetanic contraction and exercise. These observations suggest that the AMPKAR-EV mouse will pave a way to understanding the heterogeneous responses of AMPK among cell types in vivo.

リンク情報
DOI
https://doi.org/10.1016/j.celrep.2017.10.113
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/29186696
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000416979500024&DestApp=WOS_CPL
Scopus
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85035765777&origin=inward 本文へのリンクあり
Scopus Citedby
https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85035765777&origin=inward
ID情報
  • DOI : 10.1016/j.celrep.2017.10.113
  • ISSN : 2211-1247
  • eISSN : 2211-1247
  • PubMed ID : 29186696
  • SCOPUS ID : 85035765777
  • Web of Science ID : WOS:000416979500024

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