2008年10月
SuperSAGE: A Modern Platform for Genome-Wide Quantitative Transcript Profiling
CURRENT PHARMACEUTICAL BIOTECHNOLOGY
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- 巻
- 9
- 号
- 5
- 開始ページ
- 368
- 終了ページ
- 374
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.2174/138920108785915157
- 出版者・発行元
- BENTHAM SCIENCE PUBL LTD
SuperSAGE is a variant of SAGE (Serial Analysis of Gene Expression) technology, which allows making transcript profiling by 26-bp tags extracted from cDNA employing the typeIII restriction enzyme EcoP15I. Its tag length is the longest among all the versions of SAGE, and is advantageous in tag-to-gene annotation, thereby allowing the technique to applicable to any eukaryotic life organisms. For model organisms with genome or cDNA sequences available, genes corresponding to 26-bp tags are uniquely defined by simple BLAST search. For non-model organisms without these sequence information, the 26-bp tag sequence is directly applicable to design PCR primer for amplifying cDNA of corresponding genes by 3'-or 5'-RACE. Furthermore, SuperSAGE allows various applications including "interaction transcriptome" and "SuperSAGE array". Emerging "Next Generation Sequencing" technologies perfectly complement SuperSAGE, and their combination has generated a novel transcriptome platform, that is superior to all the different microarray variants in terms of throughput, data quality and cost of analysis.
- リンク情報
- ID情報
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- DOI : 10.2174/138920108785915157
- ISSN : 1389-2010
- PubMed ID : 18855689
- Web of Science ID : WOS:000260057100005