2001年2月
Production of antimicrobial defensin in Nicotiana benthamiana with a potato virus X vector
MOLECULAR PLANT-MICROBE INTERACTIONS
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- 巻
- 14
- 号
- 2
- 開始ページ
- 111
- 終了ページ
- 115
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1094/MPMI.2001.14.2.111
- 出版者・発行元
- AMER PHYTOPATHOLOGICAL SOC
A recombinant plasmid, pTXS.TH, was constructed to express the gene-encoding wasabi (Wasabia japonica) defensin with the potato virus X (PVX) vector. pTXS.TH allows the expression of defensin in the host Nicotiana benthamiana, and the defensin protein WT1 can be purified from virus-infected leaves by heat treatment and affinity chromatography. WT1 exhibits strong antifungal activity toward the phytopathogenic fungi Magnaporthe grisea (50% inhibitory concentration [IC50] = 5 mug/ml) and Botrytis cinerea (IC50 = 20 mug/ml) but is weakly active against the phytopathogenic bacterium Pseudomonas cichorii. This virus-mediated expression system is a rapid and efficient method to produce and characterize antimicrobial proteins in plants. It is particularly useful for the study of proteins that are difficult to produce with other expression systems.
- リンク情報
- ID情報
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- DOI : 10.1094/MPMI.2001.14.2.111
- ISSN : 0894-0282
- PubMed ID : 11204773
- Web of Science ID : WOS:000166511100001