論文

査読有り
2002年11月

Overexpression of the wasabi defensin gene confers enhanced resistance to blast fungus (Magnaporthe grisea) in transgenic rice

THEORETICAL AND APPLIED GENETICS
  • H Kanzaki
  • ,
  • S Nirasawa
  • ,
  • H Saitoh
  • ,
  • M Ito
  • ,
  • M Nishihara
  • ,
  • R Terauchi
  • ,
  • Nakamura, I

105
6-7
開始ページ
809
終了ページ
814
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s00122-001-0817-9
出版者・発行元
SPRINGER-VERLAG

Transgenic rice (Oryza sativa cv. Sasanishiki) overexpressing the wasabi defensin gene, a plant defensin effective against the rice blast fungus, was generated by Agrobacterium tumefaciens-mediated transformation. Twenty-two T2 homozygous lines harboring the wasabi defensin gene were challenged by the blast fungus. Transformants exhibited resistance to rice blast at various levels. The inheritance of the resistance over generations was investigated. T3 plants derived from two highly blast-resistant T2 lines (WT14-5 and WT43-5) were challenged with the blast fungus using the press-injured spots method. The average size of disease lesions of the transgenic line WT43-5 was reduced to about half of that of non-transgenic plants. The 5-kDa peptide, corresponding to the processed form of the wasabi defensin, was detected in the total protein fraction extracted from the T3 progeny. Transgenic rice plants overproducing wasabi defensin are expected to possess a durable and wide-spectrum resistance (i.e. field resistance) against various rice blast races.

リンク情報
DOI
https://doi.org/10.1007/s00122-001-0817-9
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/12582903
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000179685700001&DestApp=WOS_CPL
ID情報
  • DOI : 10.1007/s00122-001-0817-9
  • ISSN : 0040-5752
  • PubMed ID : 12582903
  • Web of Science ID : WOS:000179685700001

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