論文

査読有り 国際誌
2013年7月25日

The DHX33 RNA helicase senses cytosolic RNA and activates the NLRP3 inflammasome.

Immunity
  • Hiroki Mitoma
  • ,
  • Shino Hanabuchi
  • ,
  • Taeil Kim
  • ,
  • Musheng Bao
  • ,
  • Zhiqiang Zhang
  • ,
  • Naoshi Sugimoto
  • ,
  • Yong-Jun Liu

39
1
開始ページ
123
終了ページ
35
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.immuni.2013.07.001
出版者・発行元
CELL PRESS

The NLRP3 inflammasome plays a major role in innate immune responses by activating caspase-1, resulting in secretion of interleukin-18 (IL-18) and IL-1β. Although cytosolic double-stranded RNA (dsRNA) and bacterial RNA are known to activate the NLRP3 inflammasome, the upstream sensor is unknown. We investigated the potential function of DExD/H-box RNA helicase family members (previously shown to sense cytosolic DNA and RNA to induce type 1 interferon responses) in RNA-induced NLRP3 inflammasome activation. Among the helicase family members tested, we found that targeting of DHX33 expression by short hairpin RNA efficiently blocked the activation of caspase-1 and secretion of IL-18 and IL-1β in human macrophages that were activated by cytosolic poly I:C, reoviral RNA, or bacterial RNA. DHX33 bound dsRNA via the helicase C domain. DHX33 interacted with NLRP3 and formed the inflammasome complex following stimulation with RNA. We therefore identified DHX33 as a cytosolic RNA sensor that activates the NLRP3 inflammasome.

リンク情報
DOI
https://doi.org/10.1016/j.immuni.2013.07.001
J-GLOBAL
https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201302247178570277
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/23871209
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3756931
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000330948300013&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.immuni.2013.07.001
  • ISSN : 1074-7613
  • eISSN : 1097-4180
  • J-Global ID : 201302247178570277
  • PubMed ID : 23871209
  • PubMed Central 記事ID : PMC3756931
  • Web of Science ID : WOS:000330948300013

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