2013年7月25日
The DHX33 RNA helicase senses cytosolic RNA and activates the NLRP3 inflammasome.
Immunity
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- 巻
- 39
- 号
- 1
- 開始ページ
- 123
- 終了ページ
- 35
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.immuni.2013.07.001
- 出版者・発行元
- CELL PRESS
The NLRP3 inflammasome plays a major role in innate immune responses by activating caspase-1, resulting in secretion of interleukin-18 (IL-18) and IL-1β. Although cytosolic double-stranded RNA (dsRNA) and bacterial RNA are known to activate the NLRP3 inflammasome, the upstream sensor is unknown. We investigated the potential function of DExD/H-box RNA helicase family members (previously shown to sense cytosolic DNA and RNA to induce type 1 interferon responses) in RNA-induced NLRP3 inflammasome activation. Among the helicase family members tested, we found that targeting of DHX33 expression by short hairpin RNA efficiently blocked the activation of caspase-1 and secretion of IL-18 and IL-1β in human macrophages that were activated by cytosolic poly I:C, reoviral RNA, or bacterial RNA. DHX33 bound dsRNA via the helicase C domain. DHX33 interacted with NLRP3 and formed the inflammasome complex following stimulation with RNA. We therefore identified DHX33 as a cytosolic RNA sensor that activates the NLRP3 inflammasome.
- リンク情報
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- DOI
- https://doi.org/10.1016/j.immuni.2013.07.001
- J-GLOBAL
- https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201302247178570277
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/23871209
- PubMed Central
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3756931
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000330948300013&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1016/j.immuni.2013.07.001
- ISSN : 1074-7613
- eISSN : 1097-4180
- J-Global ID : 201302247178570277
- PubMed ID : 23871209
- PubMed Central 記事ID : PMC3756931
- Web of Science ID : WOS:000330948300013