2017年
Use of Gifu Anaerobic Medium for culturing 32 dominant species of human gut microbes and its evaluation based on short-chain fatty acids fermentation profiles
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
- 巻
- 81
- 号
- 10
- 開始ページ
- 2009
- 終了ページ
- 2017
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1080/09168451.2017.1359486
- 出版者・発行元
- TAYLOR & FRANCIS LTD
Recently, a "human gut microbial gene catalogue," which ranks the dominance of microbe genus/species in human fecal samples, was published. Most of the bacteria ranked in the catalog are currently publicly available; however, the growth media recommended by the distributors vary among species, hampering physiological comparisons among the bacteria. To address this problem, we evaluated Gifu anaerobic medium (GAM) as a standard medium. Forty-four publicly available species of the top 56 species listed in the "human gut microbial gene catalogue" were cultured in GAM, and out of these, 32 (72%) were successfully cultured. Short-chain fatty acids from the bacterial culture supernatants were then quantified, and bacterial metabolic pathways were predicted based on in silico genomic sequence analysis. Our system provides a useful platform for assessing growth properties and analyzing metabolites of dominant human gut bacteria grown in GAM and supplemented with compounds of interest.
- リンク情報
- ID情報
-
- DOI : 10.1080/09168451.2017.1359486
- ISSN : 0916-8451
- eISSN : 1347-6947
- PubMed ID : 28782454
- Web of Science ID : WOS:000413777500022