論文

査読有り 国際誌
2020年1月

Cardiomyocyte Sirt (Sirtuin) 7 Ameliorates Stress-Induced Cardiac Hypertrophy by Interacting With and Deacetylating GATA4.

Hypertension (Dallas, Tex. : 1979)
  • Satoru Yamamura
  • ,
  • Yasuhiro Izumiya
  • ,
  • Satoshi Araki
  • ,
  • Taishi Nakamura
  • ,
  • Yuichi Kimura
  • ,
  • Shinsuke Hanatani
  • ,
  • Toshihiro Yamada
  • ,
  • Toshifumi Ishida
  • ,
  • Masahiro Yamamoto
  • ,
  • Yoshiro Onoue
  • ,
  • Yuichiro Arima
  • ,
  • Eiichiro Yamamoto
  • ,
  • Yoichi Sunagawa
  • ,
  • Tatsuya Yoshizawa
  • ,
  • Naomi Nakagata
  • ,
  • Eva Bober
  • ,
  • Thomas Braun
  • ,
  • Kenji Sakamoto
  • ,
  • Koichi Kaikita
  • ,
  • Tatsuya Morimoto
  • ,
  • Kazuya Yamagata
  • ,
  • Kenichi Tsujita

75
1
開始ページ
98
終了ページ
108
記述言語
英語
掲載種別
DOI
10.1161/HYPERTENSIONAHA.119.13357

Sirt (Sirtuin) 7, the most recently identified mammalian sirtuin, has been shown to contribute to appropriate wound healing processes after acute cardiovascular insult. However, its role in the development of cardiac remodeling after pressure overload is unclear. Cardiomyocyte-specific Sirt7-knockout and control mice were subjected to pressure overload induced by transverse aortic constriction. Cardiac hypertrophy and functions were then examined in these mice. Sirt7 protein expression was increased in myocardial tissue after pressure overload. Transverse aortic constriction-induced increases in heart weight/tibial length were significantly augmented in cardiomyocyte-specific Sirt7-knockout mice compared with those of control mice. Histological analysis showed that the cardiomyocyte cross-sectional area and fibrosis area were significantly larger in cardiomyocyte-specific Sirt7-deficient mice. Cardiac contractile functions were markedly decreased in cardiomyocyte-specific Sirt7-deficient mice. Mechanistically, we found that Sirt7 interacted directly with GATA4 and that the exacerbation of phenylephrine-induced cardiac hypertrophy by Sirt7 knockdown was decreased by GATA4 knockdown. Sirt7 deacetylated GATA4 in cardiomyocytes and regulated its transcriptional activity. Interestingly, we demonstrated that treatment with nicotinamide mononucleotide, a known key NAD+ intermediate, ameliorated agonist-induced cardiac hypertrophies in a Sirt7-dependent manner in vitro. Sirt7 deficiency in cardiomyocytes promotes cardiomyocyte hypertrophy in response to pressure overload. Sirt7 exerts its antihypertrophic effect by interacting with and promoting deacetylation of GATA4.

リンク情報
DOI
https://doi.org/10.1161/HYPERTENSIONAHA.119.13357
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/31735083