論文

査読有り 筆頭著者 国際誌
2017年1月9日

Reactive sulfur species regulate tRNA methylthiolation and contribute to insulin secretion.

Nucleic acids research
  • Nozomu Takahashi
  • Fan-Yan Wei
  • Sayaka Watanabe
  • Mayumi Hirayama
  • Yuya Ohuchi
  • Atsushi Fujimura
  • Taku Kaitsuka
  • Isao Ishii
  • Tomohiro Sawa
  • Hideki Nakayama
  • Takaaki Akaike
  • Kazuhito Tomizawa
  • 全て表示

45
1
開始ページ
435
終了ページ
445
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1093/nar/gkw745

The 2-methylthio (ms2) modification at A37 of tRNAs is critical for accurate decoding, and contributes to metabolic homeostasis in mammals. However, the regulatory mechanism of ms2 modification remains largely unknown. Here, we report that cysteine hydropersulfide (CysSSH), a newly identified reactive sulfur species, is involved in ms2 modification in cells. The suppression of intracellular CysSSH production rapidly reduced ms2 modification, which was rescued by the application of an exogenous CysSSH donor. Using a unique and stable isotope-labeled CysSSH donor, we show that CysSSH was capable of specifically transferring its reactive sulfur atom to the cysteine residues of ms2-modifying enzymes as well as ms2 modification. Furthermore, the suppression of CysSSH production impaired insulin secretion and caused glucose intolerance in both a pancreatic β-cell line and mouse model. These results demonstrate that intracellular CysSSH is a novel sulfur source for ms2 modification, and that it contributes to insulin secretion.

リンク情報
DOI
https://doi.org/10.1093/nar/gkw745
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/27568003
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5224495
ID情報
  • DOI : 10.1093/nar/gkw745
  • PubMed ID : 27568003
  • PubMed Central 記事ID : PMC5224495

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