2017年5月25日
Use of RetroNectin in studies requiring in vitro HIV infection of human hematopoietic stem/progenitor cells
Journal of Virological Methods
- 巻
- 248
- 号
- 開始ページ
- 234
- 終了ページ
- 237
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1101/142075, 10.1016/j.jviromet.2017.08.003
- 出版者・発行元
- Elsevier {BV}
Human immunodeficiency virus (HIV) causes damage, directly or indirectly, to the whole hematopoietic system, including CD34+ hematopoietic stem/progenitor cells (HSPCs). CXCR4-tropic strains of HIV-1 may affect the function of CD34+CXCR4+ progenitor cells either by infecting the cells or modifying the dynamics of more differentiated hematopoietic cells. However, CD34+ cells are known for their resistance to HIV-1 infection in vitro, which restricts any detailed analysis of the impact of HIV on HSPCs. We report the use of RetroNectin, a recombinant fibronectin fragment used for gene transfer with lentiviral vectors, to overcome the limitation associated with CD34+ cell resistance to HIV-1 infection. RetroNectin coating of plates improved in vitro HIV-1 infectivity on human CD34+ cells by 10 fold. This resulted in stable HIV-1 infection for 5 weeks in an OP9-DL1 coculture. These results suggest that RetroNectin may be a useful tool for long-term monitoring of in vitro HIV-infected CD34+ cells.
- リンク情報
-
- DOI
- https://doi.org/10.1101/142075
- DOI
- https://doi.org/10.1016/j.jviromet.2017.08.003
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/28789988
- 共同研究・競争的資金等の研究課題
- HIV 感染症が造血系に与える影響の包括的解析
- 共同研究・競争的資金等の研究課題
- In vitro and in vivo analysis of the impact of human CD34+ hematopoietic stem/progenitor cells on HIV pathogenesis and latency
- ID情報
-
- DOI : 10.1101/142075
- DOI : 10.1016/j.jviromet.2017.08.003
- ORCIDのPut Code : 53293562
- PubMed ID : 28789988