論文

査読有り
2017年3月

Xeroderma pigmentosum group C protein interacts with histones: regulation by acetylated states of histone H3

GENES TO CELLS
  • Erina Kakumu
  • ,
  • Seiya Nakanishi
  • ,
  • Hiromi M. Shiratori
  • ,
  • Akari Kato
  • ,
  • Wataru Kobayashi
  • ,
  • Shinichi Machida
  • ,
  • Takeshi Yasuda
  • ,
  • Naoko Adachi
  • ,
  • Naoaki Saito
  • ,
  • Tsuyoshi Ikura
  • ,
  • Hitoshi Kurumizaka
  • ,
  • Hiroshi Kimura
  • ,
  • Masayuki Yokoi
  • ,
  • Wataru Sakai
  • ,
  • Kaoru Sugasawa

22
3
開始ページ
310
終了ページ
327
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1111/gtc.12479
出版者・発行元
WILEY

In the mammalian global genome nucleotide excision repair pathway, two damage recognition factors, XPC and UV-DDB, play pivotal roles in the initiation of the repair reaction. However, the molecular mechanisms underlying regulation of the lesion recognition process in the context of chromatin structures remain to be understood. Here, we show evidence that damage recognition factors tend to associate with chromatin regions devoid of certain types of acetylated histones. Treatment of cells with histone deacetylase inhibitors retarded recruitment of XPC to sites of UV-induced DNA damage and the subsequent repair process. Biochemical studies showed novel multifaceted interactions of XPC with histone H3, which were profoundly impaired by deletion of the N-terminal tail of histone H3. In addition, histone H1 also interacted with XPC. Importantly, acetylation of histone H3 markedly attenuated the interaction with XPC invitro, and local UV irradiation of cells decreased the level of H3K27ac in the damaged areas. Our results suggest that histone deacetylation plays a significant role in the process of DNA damage recognition for nucleotide excision repair and that the localization and functions of XPC can be regulated by acetylated states of histones.

Web of Science ® 被引用回数 : 14

リンク情報
DOI
https://doi.org/10.1111/gtc.12479
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/28233440
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000397501300006&DestApp=WOS_CPL
ID情報
  • DOI : 10.1111/gtc.12479
  • ISSN : 1356-9597
  • eISSN : 1365-2443
  • PubMed ID : 28233440
  • Web of Science ID : WOS:000397501300006

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