論文

査読有り
2016年12月

FANCI-FANCD2 stabilizes the RAD51-DNA complex by binding RAD51 and protects the 5 '-DNA end

NUCLEIC ACIDS RESEARCH
  • Koichi Sato
  • ,
  • Mayo Shimomuki
  • ,
  • Yoko Katsuki
  • ,
  • Daisuke Takahashi
  • ,
  • Wataru Kobayashi
  • ,
  • Masamichi Ishiai
  • ,
  • Hiroyuki Miyoshi
  • ,
  • Minoru Takata
  • ,
  • Hitoshi Kurumizaka

44
22
開始ページ
10758
終了ページ
10771
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1093/nar/gkw876
出版者・発行元
OXFORD UNIV PRESS

The FANCI-FANCD2 (I-D) complex is considered to work with RAD51 to protect the damaged DNA in the stalled replication fork. However, the means by which this DNA protection is accomplished have remained elusive. In the present study, we found that the I-D complex directly binds to RAD51, and stabilizes the RAD51-DNA filament. Unexpectedly, the DNA binding activity of FANCI, but not FANCD2, is explicitly required for the I-D complex-mediated RAD51-DNA filament stabilization. The RAD51 filament stabilized by the I-D complex actually protects the DNA end from nucleolytic degradation by an FA-associated nuclease, FAN1. This DNA end protection is not observed with the RAD51 mutant from FANCR patient cells. These results clearly answer the currently enigmatic question of how RAD51 functions with the I-D complex to prevent genomic instability at the stalled replication fork.

Web of Science ® 被引用回数 : 28

リンク情報
DOI
https://doi.org/10.1093/nar/gkw876
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/27694619
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000395742900021&DestApp=WOS_CPL
ID情報
  • DOI : 10.1093/nar/gkw876
  • ISSN : 0305-1048
  • eISSN : 1362-4962
  • PubMed ID : 27694619
  • Web of Science ID : WOS:000395742900021

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