論文

査読有り
2016年4月

Chromatin architecture may dictate the target site for DMC1, but not for RAD51, during homologous pairing

SCIENTIFIC REPORTS
  • Wataru Kobayashi
  • ,
  • Motoki Takaku
  • ,
  • Shinichi Machida
  • ,
  • Hiroaki Tachiwana
  • ,
  • Kazumitsu Maehara
  • ,
  • Yasuyuki Ohkawa
  • ,
  • Hitoshi Kurumizaka

6
開始ページ
24228
終了ページ
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/srep24228
出版者・発行元
NATURE PUBLISHING GROUP

In eukaryotes, genomic DNA is compacted as chromatin, in which histones and DNA form the nucleosome as the basic unit. DMC1 and RAD51 are essential eukaryotic recombinases that mediate homologous chromosome pairing during homologous recombination. However, the means by which these two recombinases distinctly function in chromatin have remained elusive. Here we found that, in chromatin, the human DMC1-single-stranded DNA complex bypasses binding to the nucleosome, and preferentially promotes homologous pairing at the nucleosome-depleted regions. Consistently, DMC1 forms ternary complex recombination intermediates with the nucleosome-free DNA or the nucleosome-depleted DNA region. Surprisingly, removal of the histone tails improperly enhances the nucleosome binding by DMC1. In contrast, RAD51 does not specifically target the nucleosome-depleted region in chromatin. These are the first demonstrations that the chromatin architecture specifies the sites to promote the homologous recombination reaction by DMC1, but not by RAD51.

Web of Science ® 被引用回数 : 8

リンク情報
DOI
https://doi.org/10.1038/srep24228
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/27052786
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000373580300001&DestApp=WOS_CPL
ID情報
  • DOI : 10.1038/srep24228
  • ISSN : 2045-2322
  • PubMed ID : 27052786
  • Web of Science ID : WOS:000373580300001

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