論文

査読有り
2014年5月

Nap1 stimulates homologous recombination by RAD51 and RAD54 in higher-ordered chromatin containing histone H1

SCIENTIFIC REPORTS
  • Shinichi Machida
  • ,
  • Motoki Takaku
  • ,
  • Masae Ikura
  • ,
  • Jiying Sun
  • ,
  • Hidekazu Suzuki
  • ,
  • Wataru Kobayashi
  • ,
  • Aiko Kinomura
  • ,
  • Akihisa Osakabe
  • ,
  • Hiroaki Tachiwana
  • ,
  • Yasunori Horikoshi
  • ,
  • Atsuhiko Fukuto
  • ,
  • Ryo Matsuda
  • ,
  • Kiyoe Ura
  • ,
  • Satoshi Tashiro
  • ,
  • Tsuyoshi Ikura
  • ,
  • Hitoshi Kurumizaka

4
開始ページ
4863
終了ページ
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/srep04863
出版者・発行元
NATURE PUBLISHING GROUP

Homologous recombination plays essential roles in mitotic DNA double strand break (DSB) repair and meiotic genetic recombination. In eukaryotes, RAD51 promotes the central homologous-pairing step during homologous recombination, but is not sufficient to overcome the reaction barrier imposed by nucleosomes. RAD54, a member of the ATP-dependent nucleosome remodeling factor family, is required to promote the RAD51-mediated homologous pairing in nucleosomal DNA. In higher eukaryotes, most nucleosomes form higher-ordered chromatin containing the linker histone H1. However, the mechanism by which RAD51/RAD54-mediated homologous pairing occurs in higher-ordered chromatin has not been elucidated. In this study, we found that a histone chaperone, Nap1, accumulates on DSB sites in human cells, and DSB repair is substantially decreased in Nap1-knockdown cells. We determined that Nap1 binds to RAD54, enhances the RAD54-mediated nucleosome remodeling by evicting histone H1, and eventually stimulates the RAD51-mediated homologous pairing in higher-ordered chromatin containing histone H1.

Web of Science ® 被引用回数 : 20

リンク情報
DOI
https://doi.org/10.1038/srep04863
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/24798879
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000335373200001&DestApp=WOS_CPL
ID情報
  • DOI : 10.1038/srep04863
  • ISSN : 2045-2322
  • PubMed ID : 24798879
  • Web of Science ID : WOS:000335373200001

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