論文

査読有り
2013年12月

Sufficient Amounts of Functional HOP2/MND1 Complex Promote Interhomolog DNA Repair but Are Dispensable for Intersister DNA Repair during Meiosis in Arabidopsis

PLANT CELL
  • Clemens Uanschou
  • ,
  • Arnaud Ronceret
  • ,
  • Mona Von Harder
  • ,
  • Arnaud De Muyt
  • ,
  • Daniel Vezon
  • ,
  • Lucie Pereira
  • ,
  • Liudmila Chelysheva
  • ,
  • Wataru Kobayashi
  • ,
  • Hitoshi Kurumizaka
  • ,
  • Peter Schloegelhofer
  • ,
  • Mathilde Grelon

25
12
開始ページ
4924
終了ページ
4940
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1105/tpc.113.118521
出版者・発行元
AMER SOC PLANT BIOLOGISTS

During meiosis, homologous recombination (HR) is essential to repair programmed DNA double-strand breaks (DSBs), and a dedicated protein machinery ensures that the homologous chromosome is favored over the nearby sister chromatid as a repair template. The HOMOLOGOUS-PAIRING PROTEIN2/MEIOTIC NUCLEAR DIVISION PROTEIN1 (HOP2/MND1) protein complex has been identified as a crucial factor of meiotic HR in Arabidopsis thaliana, since loss of either MND1 or HOP2 results in failure of DNA repair. We isolated two mutant alleles of HOP2 (hop2-2 and hop2-3) that retained the capacity to repair meiotic DSBs via the sister chromatid but failed to use the homologous chromosome. We show that in these alleles, the recombinases RADIATION SENSITIVE51 (RAD51) and DISRUPTED MEIOTIC cDNA1 (DMC1) are loaded, but only the intersister DNA repair pathway is activated. The hop2-2 phenotype is correlated with a decrease in HOP2/MND1 complex abundance. In hop2-3, a truncated HOP2 protein is produced that retains its ability to bind to DMC1 and DNA but forms less stable complexes with MND1 and fails to efficiently stimulate DMC1-driven D-loop formation. Genetic analyses demonstrated that in the absence of DMC1, HOP2/MND1 is dispensable for RAD51-mediated intersister DNA repair, while in the presence of DMC1, a minimal amount of functional HOP2/MND1 is essential to drive intersister DNA repair.

Web of Science ® 被引用回数 : 27

リンク情報
DOI
https://doi.org/10.1105/tpc.113.118521
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/24363313
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000330611200016&DestApp=WOS_CPL
ID情報
  • DOI : 10.1105/tpc.113.118521
  • ISSN : 1040-4651
  • eISSN : 1532-298X
  • PubMed ID : 24363313
  • Web of Science ID : WOS:000330611200016

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