論文

査読有り 国際誌
2020年1月7日

Cryo-EM Structures of Centromeric Tri-nucleosomes Containing a Central CENP-A Nucleosome.

Structure (London, England : 1993)
  • Yoshimasa Takizawa
  • ,
  • Cheng-Han Ho
  • ,
  • Hiroaki Tachiwana
  • ,
  • Hideyuki Matsunami
  • ,
  • Wataru Kobayashi
  • ,
  • Midori Suzuki
  • ,
  • Yasuhiro Arimura
  • ,
  • Tetsuya Hori
  • ,
  • Tatsuo Fukagawa
  • ,
  • Melanie D Ohi
  • ,
  • Matthias Wolf
  • ,
  • Hitoshi Kurumizaka

28
1
開始ページ
44
終了ページ
53
記述言語
英語
掲載種別
DOI
10.1016/j.str.2019.10.016

The histone H3 variant CENP-A is a crucial epigenetic marker for centromere specification. CENP-A forms a characteristic nucleosome and dictates the higher-order configuration of centromeric chromatin. However, little is known about how the CENP-A nucleosome affects the architecture of centromeric chromatin. In this study, we reconstituted tri-nucleosomes mimicking a centromeric nucleosome arrangement containing the CENP-A nucleosome, and determined their 3D structures by cryoelectron microscopy. The H3-CENP-A-H3 tri-nucleosomes adopt an untwisted architecture, with an outward-facing linker DNA path between nucleosomes. This is distinct from the H3-H3-H3 tri-nucleosome architecture, with an inward-facing DNA path. Intriguingly, the untwisted architecture may allow the CENP-A nucleosome to be exposed to the solvent in the condensed chromatin model. These results provide a structural basis for understanding the 3D configuration of CENP-A-containing chromatin, and may explain how centromeric proteins can specifically target the CENP-A nucleosomes buried in robust amounts of H3 nucleosomes in centromeres.

リンク情報
DOI
https://doi.org/10.1016/j.str.2019.10.016
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/31711756
ID情報
  • DOI : 10.1016/j.str.2019.10.016
  • ISSN : 0969-2126
  • PubMed ID : 31711756

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