論文

査読有り
2017年12月

Electrostatic balance between global repulsion and local attraction in reentrant polymerization of actin

CYTOSKELETON
  • Jun Ohnuki
  • ,
  • Akira Yodogawa
  • ,
  • Mitsunori Takano

74
12
開始ページ
504
終了ページ
511
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1002/cm.21391
出版者・発行元
WILEY

Actin polymerization depends on the salt concentration, exhibiting a reentrant behavior: the polymerization is promoted by increasing KCl concentration up to 100 mM, and then depressed by further increase above 100 mM. We here investigated the physical mechanism of this reentrant behavior by calculating the polymerization energy, defined by the electrostatic energy change upon binding of an actin subunit to a filament, using an implicit solvent model based on the Poisson-Boltzmann (PB) equation. We found that the polymerization energy as a function of the salt concentration shows a non-monotonic reentrant-like behavior, with the minimum at about 100 mM (1:1 salt). By separately examining the salt concentration effect on the global electrostatic repulsion between the like-charged subunits and that on the local electrostatic attraction between the inter-subunit ionic-bond-forming residues in the filament, we clarified that the reentrant behavior is caused by the change in the balance between the two opposing electrostatic interactions. Our study showed that the non-specific nature of counterions, as described in the mean-field theory, plays an important role in the actin polymerization. We also discussed the endothermic nature of the actin polymerization and mentioned the effect of ATP hydrolysis on the G-F transformation, indicating that the electrostatic interaction is widely and intricately involved in the actin dynamics.

リンク情報
DOI
https://doi.org/10.1002/cm.21391
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000417746200008&DestApp=WOS_CPL
ID情報
  • DOI : 10.1002/cm.21391
  • ISSN : 1949-3584
  • eISSN : 1949-3592
  • Web of Science ID : WOS:000417746200008

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