論文

2020年10月31日

Circadian key component CLOCK/BMAL1 interferes with segmentation clock in mouse embryonic organoids

  • Yasuhiro Umemura
  • ,
  • Nobuya Koike
  • ,
  • Yoshiki Tsuchiya
  • ,
  • Hitomi Watanabe
  • ,
  • Gen Kondoh
  • ,
  • Ryoichiro Kageyama
  • ,
  • Kazuhiro Yagita

DOI
10.1101/2020.10.30.362830
出版者・発行元
Cold Spring Harbor Laboratory

<title>Abstract</title>In mammals, circadian clocks are strictly suppressed during early embryonic stages as well as pluripotent stem cells, by the lack of CLOCK/BMAL1 mediated circadian feedback loops. During ontogenesis, the innate circadian clocks emerge gradually at a late developmental stage, then, with which the circadian temporal order is invested in each cell level throughout a body. Meanwhile, in the early developmental stage, a segmented body plan is essential for an intact developmental process and somitogenesis is controlled by another cell-autonomous oscillator, the segmentation clock, in the posterior presomitic mesoderm (PSM). In the present study, focusing upon the interaction between circadian key components and the segmentation clock, we investigated the effect of the CLOCK/BMAL1 on the segmentation clock <italic>Hes7</italic> oscillation, revealing that the expression of functional CLOCK/BMAL1 severely interferes with the ultradian rhythm of segmentation clock in induced PSM and gastruloids. RNA sequencing analysis showed that the premature expression of CLOCK/BMAL1 affects the <italic>Hes7</italic> transcription and its regulatory pathways. These results suggest that the suppression of CLOCK/BMAL1-mediated transcriptional regulation during the somitogenesis may be inevitable for intact mammalian development.

リンク情報
DOI
https://doi.org/10.1101/2020.10.30.362830
URL
https://syndication.highwire.org/content/doi/10.1101/2020.10.30.362830
ID情報
  • DOI : 10.1101/2020.10.30.362830

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