論文

査読有り
1995年9月

GENERATION AND CHARACTERIZATION OF A HOST CELL-DEPENDENT GAG GENE MUTANT OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1

VIROLOGY
  • J SAKURAGI
  • ,
  • H SAKAI
  • ,
  • M KAWAMURA
  • ,
  • K TOKUNAGA
  • ,
  • S UEDA
  • ,
  • A ADACHI

212
1
開始ページ
251
終了ページ
254
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1006/viro.1995.1478
出版者・発行元
ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS

An in-frame gag gene mutant of human immunodeficiency virus type 1, which carries two amino acid substitutions in the center of the p24 coding region, was constructed in vitro, and its replication properties in several cell lines were examined. In CD4-negative SW480 cells transfected with the mutant clone, synthesis and processing of viral gag, pol, and env proteins occurred normally, and viral particles were produced. Virions derived from the transfection displayed a severe replication defect when inoculated into some CD4-positive cell lines (H9 and Molt4 clone 8), but in other lines (A3.01 and M8166), the mutant virus grew fairly well. The mutant was demonstrated to be defective at an early infection phase (from adsorption to integration) in Molt4, clone 8 cells but was normal in A3.01 cells. These results indicated that the Gag-p24 protein of human immunodeficiency virus type 1 plays an important role at the early infection phase in a cell-dependent manner. (C) 1995 Academic Press, Inc.

リンク情報
DOI
https://doi.org/10.1006/viro.1995.1478
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:A1995RV17800032&DestApp=WOS_CPL
ID情報
  • DOI : 10.1006/viro.1995.1478
  • ISSN : 0042-6822
  • Web of Science ID : WOS:A1995RV17800032

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