Toshiaki Watanabe

J-GLOBAL         Last updated: Jun 8, 2019 at 00:46
 
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Name
Toshiaki Watanabe
Affiliation
Central Institute for Experimental Animals

Research Areas

 
 

Academic & Professional Experience

 
Apr 2017
 - 
Today
Researcher, Department of marmoset biology and medicine, Central Institute for Experimental Animals
 
Oct 2016
 - 
Mar 2017
Assistant Professor, School of Medicine, Kyoto Univeristy
 
Mar 2010
 - 
Sep 2016
Postdoctoral researcher, Yale University
 
Apr 2009
 - 
Feb 2010
Researcher, National Institute of Genetics
 

Education

 
 
 - 
Mar 2009
Genetics, Bioogical science, SOKENDAI
 
 
 - 
Mar 2006
Applied Biology, School of agriculture, Kyoto University
 
 
 - 
Mar 2003
Biophhysics and Biochemistry, School of Science, The University of Tokyo
 

Published Papers

 
Watanabe T, Yamazaki S, Yoneda N, Shinohara H, Tomioka I, Higuchi Y, Yagoto M, Ema M, Suemizu H, Kawai K, Sasaki E
Genes to cells : devoted to molecular & cellular mechanisms      May 2019   [Refereed]
Toshiaki Watanabe, Xiekui Cui, Zhongyu Yuan, Hongying Qi, Haifan Lin
EMBO Journal   37    Sep 2018
© 2018 The Authors Argonaute/Piwi proteins can regulate gene expression via RNA degradation and translational regulation using small RNAs as guides. They also promote the establishment of suppressive epigenetic marks on repeat sequences in diverse...
Takuji Yoshimura, Toshiaki Watanabe, Satomi Kuramochi-Miyagawa, Noriaki Takemoto, Yusuke Shiromoto, Akihiko Kudo, Masami Kanai-Azuma, Fumi Tashiro, Satsuki Miyazaki, Ami Katanaya, Shinichiro Chuma, Jun Ichi Miyazaki
EMBO Reports   19    Apr 2018
© 2018 The Authors The piRNA pathway is a piRNA-guided retrotransposon silencing system which includes processing of retrotransposon transcripts by PIWI-piRNAs in secondary piRNA biogenesis. Although several proteins participate in the piRNA pathw...
Toshiaki Watanabe, Ee Chun Cheng, Mei Zhong, Haifan Lin
Genome Research   25 368-380   Jan 2015
© 2015 Watanabe et al. The eukaryotic genome has vast intergenic regions containing transposons, pseudogenes, and other repetitive sequences. They produce numerous long noncoding RNAs (lncRNAs) and Piwi-interacting RNAs (piRNAs), yet the functions...
Toshiaki Watanabe, Haifan Lin
Molecular Cell   56 18-27   Jan 2014
© 2014 Elsevier Inc. Piwi proteins and Piwi-interacting RNAs (piRNAs) are essential for gametogenesis, embryogenesis, and stem cell maintenance in animals. Piwi proteins act on transposon RNAs by cleaving the RNAs and by interacting with factors i...
Reina Komiya, Hajime Ohyanagi, Mitsuru Niihama, Toshiaki Watanabe, Mutsuko Nakano, Nori Kurata, Ken Ichi Nonomura
Plant Journal   78 385-397   Jan 2014
Small RNAs that interact with Argonaute (AGO) proteins play central roles in RNA-mediated silencing. MEIOSIS ARRESTED AT LEPTOTENE1 (MEL1), a rice AGO, has specific functions in the development of pre-meiotic germ cells and the progression of meio...
Yasuhiro Yamamoto, Toshiaki Watanabe, Yuko Hoki, Kenjiro Shirane, Yufeng Li, Kenji Ichiiyanagi, Satomi Kuramochi-Miyagawa, Atsushi Toyoda, Asao Fujiyama, Masayuki Oginuma, Hitomi Suzuki, Takashi Sado, Toru Nakano, Hiroyuki Sasaki
Genome Research   23 292-299   Feb 2013
In germ cells, early embryos, and stem cells of animals, PIWI-interacting RNAs (piRNAs) have an important role in silencing retrotransposons, which are vicious genomic parasites, through transcriptional and post-transcriptional mechanisms. To exam...
Nicole Darricarrére, Na Liu, Toshiaki Watanabe, Haifan Lin
Proceedings of the National Academy of Sciences of the United States of America   110 1297-1302   Jan 2013
The Piwi protein subfamily is essential for Piwi-interacting RNA (piRNA) biogenesis, transposon silencing, and germ-line development, all ofwhich have been proposed to require Piwi endonuclease activity, as validated for two cytoplasmic Piwi prote...
Kenji Ichiyanagi, Yufeng Li, Toshiaki Watanabe, Tomoko Ichiyanagi, Kei Fukuda, Junko Kitayama, Yasuhiro Yamamoto, Satomi Kuramochi-Miyagawa, Toru Nakano, Yukihiro Yabuta, Yoshiyuki Seki, Mitinori Saitou, Hiroyuki Sasaki
Genome Research   22 810   Apr 2012
Yusuke Ohnishi, Yasushi Totoki, Atsushi Toyoda, Toshiaki Watanabe, Yasuhiro Yamamoto, Katsushi Tokunaga, Yoshiyuki Sakaki, Hiroyuki Sasaki, Hirohiko Hohjoh
Molecular Biology Reports   39 903-909   Feb 2012
Small RNAs derived from repetitive sequences appear to play essential roles in mammalian gametogenesis and early development. In this study we focused on the short interspersed nucleotide element B1 (SINE/B1) small RNAs, which were zygotically exp...
Kenji Ichiyanagi, Yungfeng Li, Toshiaki Watanabe, Tomoko Ichiyanagi, Kei Fukuda, Junko Kitayama, Yasuhiro Yamamoto, Satomi Kuramochi-Miyagawa, Toru Nakano, Yukihiro Yabuta, Yoshiyuki Seki, Mitinori Saitou, Hiroyuki Sasaki
Genome Research   21 2058-2066   Dec 2011
In mammals, germ cells undergo striking dynamic changes in DNA methylation during their development. However, the dynamics and mode of methylation are poorly understood for short interspersed elements (SINEs) dispersed throughout the genome. We in...
Toshiaki Watanabe, Shin Ichi Tomizawa, Kohzoh Mitsuya, Yasushi Totoki, Yasuhiro Yamamoto, Satomi Kuramochi-Miyagawa, Naoko Iida, Yuko Hoki, Patrick J. Murphy, Atsushi Toyoda, Kengo Gotoh, Hitoshi Hiura, Takahiro Arima, Asao Fujiyama, Takashi Sado, Tatsuhiro Shibata, Toru Nakano, Haifan Lin, Kenji Ichiyanagi, Paul D. Soloway, Hiroyuki Sasaki
Science   332 848-852   May 2011
Genomic imprinting causes parental origin - specific monoallelic gene expression through differential DNA methylation established in the parental germ line. However, the mechanisms underlying how specific sequences are selectively methylated are n...
Toshiaki Watanabe, Shinichiro Chuma, Yasuhiro Yamamoto, Satomi Kuramochi-Miyagawa, Yasushi Totoki, Atsushi Toyoda, Yuko Hoki, Asao Fujiyama, Tatsuhiro Shibata, Takashi Sado, Toshiaki Noce, Toru Nakano, Norio Nakatsuji, Haifan Lin, Hiroyuki Sasaki
Developmental Cell   20 364-375   Mar 2011
MITOPLD is a member of the phospholipase D superfamily proteins conserved among diverse species. Zucchini (Zuc), the Drosophila homolog of MITOPLD, has been implicated in primary biogenesis of Piwi-interacting RNAs (piRNAs). By contrast, MITOPLD h...
Shin Ichi Tomizawa, Hisato Kobayashi, Toshiaki Watanabe, Simon Andrews, Kenichiro Hata, Gavin Kelsey, Hiroyuki Sasaki
Development   138 811-820   Mar 2011
Mammalian imprinted genes are associated with differentially methylated regions (DMRs) that are CpG methylated on one of the two parental chromosomes. In mice, at least 21 DMRs acquire differential methylation in the germline and many of them act ...
Hongying Qi, Toshiaki Watanabe, Hsueh Yen Ku, Na Liu, Mei Zhong, Haifan Lin
Journal of Biological Chemistry   286 3789-3797   Feb 2011
Despite exciting progress in understanding the Piwi-interacting RNA (piRNA) pathway in the germ line, less is known about this pathway in somatic cells. We showed previously that Piwi, a key component of the piRNA pathway in Drosophila, is regulat...
Satomi Kuramochi-Miyagawa, Toshiaki Watanabe, Kengo Gotoh, Kana Takamatsu, Shinichiro Chuma, Kanako Kojima-Kita, Yusuke Shiromoto, Noriko Asada, Atsushi Toyoda, Asao Fujiyama, Yasushi Totoki, Tatsuhiro Shibata, Tohru Kimura, Norio Nakatsuji, Toshiaki Noce, Hiroyuki Sasaki, Toru Nakano
Genes and Development   24 887-892   May 2010
VASA is an evolutionarily conserved RNA helicase essential for germ cell development. The mouse PIWI family proteins MILI and MIWI2 are involved in production of Piwi-interacting RNAs (piRNAs) in fetal male germ cells through a ping-pong amplifica...
Yusuke Ohnishi, Yasushi Totoki, Atsushi Toyoda, Toshiaki Watanabe, Yasuhiro Yamamoto, Katsushi Tokunaga, Yoshiyuki Sakaki, Hiroyuki Sasaki, Hirohiko Hohjoh
Nucleic Acids Research   38 5141-5151   Apr 2010
Recent studies showed that small interfering RNAs (siRNAs) and Piwi-interacting RNA (piRNA) in mammalian germ cells play important roles in retrotransposon silencing and gametogenesis. However, subsequent contribution of those small RNAs to early ...
Toshiaki Watanabe, Yasushi Totoki, Atsushi Toyoda, Masahiro Kaneda, Satomi Kuramochi-Miyagawa, Yayoi Obata, Hatsune Chiba, Yuji Kohara, Tomohiro Kono, Toru Nakano, M. Azim Surani, Yoshiyuki Sakaki, Hiroyuki Sasaki
Nature   453 539-543   May 2008
RNA interference (RNAi) is a mechanism by which double-stranded RNAs (dsRNAs) suppress specific transcripts in a sequence-dependent manner. dsRNAs are processed by Dicer to 21-24-nucleotide small interfering RNAs (siRNAs) and then incorporated int...
Atsushi Takeda, Shintaro Iwasaki, Toshiaki Watanabe, Maki Utsumi, Yuichiro Watanabe
Plant and Cell Physiology   49 493-500   Apr 2008
Double-stranded RNA induces RNA silencing and is cleaved into 21-24 nt small RNA duplexes by Dicer enzyme. A strand of Dicer-generated small RNA duplex (called the guide strand) is then selected by a thermodynamic mechanism to associate with Argon...
Satomi Kuramochi-Miyagawa, Toshiaki Watanabe, Kengo Gotoh, Yasushi Totoki, Atsushi Toyoda, Masahito Ikawa, Noriko Asada, Kanako Kojima, Yuka Yamaguchi, Takashi W. Ijiri, Kenichiro Hata, En Li, Yoichi Matsuda, Tohru Kimura, Masaru Okabe, Yoshiyuki Sakaki, Hiroyuki Sasaki, Toru Nakano
Genes and Development   22 908-917   Apr 2008
Silencing of transposable elements occurs during fetal gametogenesis in males via de novo DNA methylation of their regulatory regions. The loss of MILI (miwi-like) and MIWI2 (mouse piwi 2), two mouse homologs of Drosophila Piwi, activates retrotra...
Toshiaki Watanabe, Hiroshi Imai, Naojiro Minami
Methods in Molecular Biology   442 173-185   Jan 2008
RNA interference (RNAi) is a sequence-specific gene regulatory mechanism in which the specificity is determined by small RNAs. Three major classes of endogenous small RNAs, namely microRNAs (miRNAs), small interfering RNAs (siRNAs), and piwi-inter...
Toshiaki Watanabe, Yasushi Totoki, Hiroyuki Sasaki, Naojiro Minami, Hiroshi Imai
Methods in Enzymology   427 155-169   Dec 2007
Small RNAs ranging in size between 20 and 32 nt regulate gene expression through chromatin modification, mRNA degradation, and translational repression. Three major classes of small RNAs have been characterized: microRNAs (miRNAs), short interferi...
Toshiaki Watanabe, Atsushi Takeda, Tomoyuki Tsukiyama, Kazuyuki Mise, Tetsuro Okuno, Hiroyuki Sasaki, Naojiro Minami, Hiroshi Imai
Genes and Development   20 1732-1743   Jul 2006
Small RNAs ranging in size between 18 and 30 nucleotides (nt) are found in many organisms including yeasts, plants, and animals. Small RNAs are involved in the regulation of gene expression through translational repression, mRNA degradation, and c...
Toshiaki Watanabe, Atsushi Takeda, Kazuyuki Mise, Tetsuro Okuno, Toru Suzuki, Naojiro Minami, Hiroshi Imai
FEBS Letters   579 318-324   Jan 2005
MicroRNAs (miRNAs) repress target genes at the post-transcriptional level and play important roles in development and cell lineage decision. However, in vertebrates, both the targets of miRNAs and their expression profile during development are po...