論文

査読有り
2013年12月

A device for the rapid transfer/transplantation of living cell sheets with the absence of cell damage

Biomaterials
  • Kenjiro Tadakuma
  • ,
  • Nobuyuki Tanaka
  • ,
  • Yuji Haraguchi
  • ,
  • Mitsuru Higashimori
  • ,
  • Makoto Kaneko
  • ,
  • Tatsuya Shimizu
  • ,
  • Masayuki Yamato
  • ,
  • Teruo Okano

34
36
開始ページ
9018
終了ページ
9025
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.biomaterials.2013.08.006

In this study, we developed a device that could easily, rapidly, and completely transfer cell sheets from one material to another or transplant cell sheets onto the dorsal subcutaneous tissues of rats without leaving residual cells. Because the manipulation is as simple as pipetting, technical expertise is not required to transfer cell sheets very rapidly (the transfer time was 3.7±1.6s) using the device compared with that of a conventional method using a pipette (430±180s). After transfer by the device, C2C12 skeletal myoblast sheets showed active cell metabolism, cell viability, and very high production of vascular endothelial growth factor and stromal-derived factor-1α, indicating transfer without cell damage. Cardiac cell sheets after transfer showed spontaneous and synchronous beating, indicating intact cell-cell junctions and ion channel proteins on the cell surface. In addition, the device allowed us to transfer C2C12 cell sheets onto soft, rugged and curved surfaces such as human hands. Furthermore, cardiac cell sheets adhered rapidly and tightly onto the dorsal subcutaneous tissues of rats. This transfer/transplantation device may be a powerful tool in cell sheet-based tissue engineering and regenerative medicine. © 2013 Elsevier Ltd.

リンク情報
DOI
https://doi.org/10.1016/j.biomaterials.2013.08.006
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/23972478
ID情報
  • DOI : 10.1016/j.biomaterials.2013.08.006
  • ISSN : 0142-9612
  • ISSN : 1878-5905
  • PubMed ID : 23972478
  • SCOPUS ID : 84884202504

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