論文

査読有り
2015年7月25日

Effects of Hemagglutination Activity in the Serum of a Deep-Sea Vent Endemic Crab, Shinkaia Crosnieri, on Non-Symbiotic and Symbiotic Bacteria.

Microbes and environments
  • So Fujiyoshi
  • ,
  • Hiroaki Tateno
  • ,
  • Tomoo Watsuji
  • ,
  • Hideyuki Yamaguchi
  • ,
  • Daisuke Fukushima
  • ,
  • Sayaka Mino
  • ,
  • Makoto Sugimura
  • ,
  • Tomoo Sawabe
  • ,
  • Ken Takai
  • ,
  • Shigeki Sawayama
  • ,
  • Satoshi Nakagawa

担当区分
筆頭著者
記述言語
掲載種別
研究論文(学術雑誌)
DOI
10.1264/jsme2.me15066

In deep-sea hydrothermal environments, most invertebrates associate with dense populations of symbiotic microorganisms in order to obtain nutrition. The molecular interactions between deep-sea animals and environmental microbes, including their symbionts, have not yet been elucidated in detail. Hemagglutinins/lectins, which are carbohydrate-binding proteins, have recently been reported to play important roles in a wide array of biological processes, including the recognition and control of non-self materials. We herein assessed hemagglutination activity in the serum of a deep-sea vent endemic crab, Shinkaia crosnieri, which harbors chemosynthetic epibionts on its plumose setae. Horse and rabbit erythrocytes were agglutinated using this serum (opt. pH 7.5 and opt. temperature 15°C). Agglutinating activity was inhibited by eight kinds of sugars and several divalent cations, did not require any divalent metal ions, and remained detectable even after heating the serum at 100°C for 30 min. By using fluorescently labeled serum, we demonstrated that deep-sea crab serum components bound to the epibionts even in the presence of sugars. This study represents the first immunological assessment of a deep-sea vent endemic crab and demonstrated the possibility of a non-lectin-mediated symbiont-host interaction.

リンク情報
DOI
https://doi.org/10.1264/jsme2.me15066
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/26212518
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4567561
URL
https://europepmc.org/articles/PMC4567561

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