Papers

Peer-reviewed
1994

CLONING AND CHARACTERIZATION OF HUMAN TYPE-2 AND TYPE-3 INOSITOL 1,4,5-TRISPHOSPHATE RECEPTORS

RECEPTORS & CHANNELS
  • M YAMAMOTOHINO
  • T SUGIYAMA
  • K HIKICHI
  • MG MATTEI
  • K HASEGAWA
  • S SEKINE
  • K SAKURADA
  • A MIYAWAKI
  • T FURUICHI
  • M HASEGAWA
  • K MIKOSHIBA
  • Display all

Volume
2
Number
1
First page
9
Last page
22
Language
English
Publishing type
Research paper (scientific journal)
Publisher
HARWOOD ACAD PUBL GMBH

We have cloned cDNAs coding for human type 2 and type 3 and part of type 1 inositol 1,4,5-trisphosphate receptors (IP(3)Rs). The complete nucleotide sequences for type 2 and type 3 receptors were determined and the pharmacological properties of the latter were characterized. Human type 2 and type 3 IP(3)Rs are 2701 amino acids and 2671 amino acids long, respectively, and have significant sequence homologies as well as structural similarities including the six membrane-spanning regions near the C-termini when compared with the rat or mouse counterpart. COS-7 cells transfected with human type 3 IP(3)R showed characteristic inositol 1,4,5-trisphosphate (IP3)-binding properties with Kd values of 28.8 nM. The order of potency of competition with IP3 was Ins(1,4,5)P-3 (IP3) > Ins(2,4,5)P-3 > Ins(1,3,4,5)P-4 > Ins(1,2,3,4,5,6)P-6. Type 2 and type 3 IP(3)Rs were mapped to human chromosomes 12p11 and 6p21, respectively, by in situ hybridization. cDNA cloning of the human IP(3)Rs allowed us to identify the types of the receptor expressed in various human hematopoietic and lymphoma cell lines. The type 3 receptor was present in all of cell lines tested, while the type 1 or 2 receptor was expressed in only particular cell types. The diffential expression of the IP(3)R types could confer the cell-specific regulation on the IP3/Ca2+ signalling.

Link information
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:A1994NT28300002&DestApp=WOS_CPL
ID information
  • ISSN : 1060-6823
  • Web of Science ID : WOS:A1994NT28300002

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