2020年6月
Integral approach to biomacromolecular structure by analytical-ultracentrifugation and small-angle scattering
Communications Biology
- 巻
- 3
- 号
- 1
- 開始ページ
- 294
- 終了ページ
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1038/s42003-020-1011-4
- 出版者・発行元
- Springer Science and Business Media LLC
<title>Abstract</title>Currently, a sample for small-angle scattering (SAS) is usually highly purified and looks monodispersed: The Guinier plot of its SAS intensity shows a fine straight line. However, it could include the slight aggregates which make the experimental SAS profile different from the monodispersed one. A concerted method with analytical-ultracentrifugation (AUC) and SAS, named as AUC-SAS, offers the precise scattering intensity of a concerned biomacromolecule in solution even with aggregates as well that of a complex under an association-dissociation equilibrium. AUC-SAS overcomes an aggregation problem which has been an obstacle for SAS analysis and, furthermore, has a potential to lead to a structural analysis for a general multi-component system.
- リンク情報
- ID情報
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- DOI : 10.1038/s42003-020-1011-4
- eISSN : 2399-3642