J-GLOBAL         Last updated: Aug 31, 2019 at 02:51
Showa Women's University
Faculty of Life and Environmental Sciences, Department of Health Science

Research Areas



Advanced Food Preservation by Hurdle Technology and the Application of Predictive Microbiology
(926) 49-55   Dec 2017   [Refereed]
A modified single-cell electroporation method for molecule delivery into a motile protest, Euglena gracilis.
◎Ohmachi M., Fujiwara Y., Muramatsu S., Yamada K., Iwata O., Suzuki K., and Wang O.
Journal of Microbiological Methods   130 106-111   Nov 2016   [Refereed]
Single-cell transfection is a powerful technique for delivering chemicals, drugs, or probes into arbitrary, specific single cells. This technique is especially important when the analysis of molecular function and cellular behavior in individual m...
Expression level of methanol-inducible peroxisomal proteins and peroxisome morphology are affected by oxygen conditions and mitochondrial respiratory pathway function in the methylotrophic yeast Candida boidinii.
◎Fujimura S., Yurimoto H., Kurimoto S., Matsufuji Y., Ito T., Hayakawa T., Tomizuka N., Sakai Y. and Nakagawa T.
FEMS Yeast Research   13(4) 359-366   Jun 2013   [Refereed]
In the methylotrophic yeast, Candida boidinii, methanol-inducible peroxisomal proteins, for example alcohol oxidase (AOD), dihydroxyacetone synthase (DAS), and peroxisomal glutathione peroxidase (Pmp20), were induced only under aerobic cond...
Alteration of ethanol tolerance caused by the deficiency in the genes associated with histone deacetylase complex in budding yeast.
◎Matsuda T., Fujimura S., Suda H., Matsufuji Y., and Nakagawa J.
Bioscience, Biotechnology, and Biochemistry   75(9) 1829-1831   Sep 2011   [Refereed]
Upon exposure to 8% ethanol, survival and growth of yeast strains deficient in histone deacetylase complex genes was examined. Of the 18 mutants tested, the Δsir3 and Δsir4 strains showed higher resistance to ethanol, while the Δr...
Transcription factor Stb5p is essential for acetoaldehyde tolerance in Saccharomyces cerevisiae.
◎Matsufuji, Y., Nakagawa, T., Fujimura, S., Tani, A. and Nakagawa, J.
Journal of Basic Microbiology   50(5) 494-498   Oct 2010   [Refereed]
Transcription factor Stb5p, previously known as one of the multidrug resistance gene regulators in Saccharomyces cerevisiae, was shown here to play an essential role in acetaldehyde tolerance. A mutant strain, Δstb5 exhibited increased acet...

Books etc

Food hygiene
Jan 2019   
Current Topics in Biochemistry Regulation of methanol metabolism in methylotrophic yeast Pichia methanolica.
Consultant editor, Ohyama, T. ◎Nakagawa, T., Fujimura, S., Ito, T., Miyaji, T. and Tomizuka, N. (Part:Joint Work, Regulation of methanol metabolism in methylotrophic yeast <I>Pichia methanolica</I>.)
Research Signpost, India   May 2006   

Conference Activities & Talks

Epigenetic regulation involved in ethanol stress response in Saccharomyces cerevisiae.
Fujimura S., Matsuda T., Suda H. and Nakagawa J.
Yeast Genetics & Molecular Biology Meeting   Jul 2012   
Eukaryotic cells encounter various environmental stresses that can cause growth inhibition or cell death. During fermentation process, yeast cells are exposed to various stresses such as oxidative, high sugar content, starvation and ethanol. Ethan...
The regulatory system of methanol metabolism by oxygen in methylotrophic yeast Pichia methanolica.
Fujimura S., Nakagawa T., Matsufuji Y., Miyaji T., Tomizuka N. and Nakagawa J.
XXIVth International Conference on Yeast Genetics and Molecular Biology.   Jul 2009   
In Pichia methanolica, expression of methanol-metabolic enzymes was strictly regulated by the presence of oxygen, and induction of alcohol oxidase (AOD) isozymes was largely dependent on oxygen concentrations. The proportion of AOD-isozyme species...
Peroxisomal metabolism is regulated by oxygen-recognition system in methylitorophic yeast Pichia methanolica.
Fujimura S., Nakagawa T., Ito T., Matsufuji Y., Miyaji T. and Tomizuka N.
XXIIIrd International Conference on Yeast Genetics and Molecular Biology   Jul 2007   
In Pichia methanolica, expressions of methanol-metabolic enzymes were strictly regulated by the presence of oxygen, and induction of alcohol oxidase (AOD) isozymes was completely dependent on oxygen concentrations. ... These indicated that P. meth...