2013年6月
Improved computational target site prediction for pentatricopeptide repeat RNA editing factors.
PloS one
- ,
- ,
- ,
- 巻
- 8
- 号
- 6
- 開始ページ
- e65343
- 終了ページ
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1371/journal.pone.0065343
- 出版者・発行元
- PUBLIC LIBRARY SCIENCE
Pentatricopeptide repeat (PPR) proteins with an E domain have been identified as specific factors for C to U RNA editing in plant organelles. These PPR proteins bind to a unique sequence motif 59 of their target editing sites. Recently, involvement of a combinatorial amino acid code in the P (normal length) and S type (short) PPR domains in sequence specific RNA binding was reported. PPR proteins involved in RNA editing, however, contain not only P and S motifs but also their long variants L (long) and L2 (long2) and the S2 (short2) motifs. We now find that inclusion of these motifs improves the prediction of RNA editing target sites. Previously overlooked RNA editing target sites are suggested from the PPR motif structures of known E-class PPR proteins and are experimentally verified. RNA editing target sites are assigned for the novel PPR protein MEF32 (mitochondrial editing factor 32) and are confirmed in the cDNA.
- リンク情報
-
- DOI
- https://doi.org/10.1371/journal.pone.0065343
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/23762347
- PubMed Central
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3675099
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000321099000057&DestApp=WOS_CPL
- URL
- http://europepmc.org/abstract/med/23762347
- ID情報
-
- DOI : 10.1371/journal.pone.0065343
- ISSN : 1932-6203
- ORCIDのPut Code : 88066520
- PubMed ID : 23762347
- PubMed Central 記事ID : PMC3675099
- Web of Science ID : WOS:000321099000057