2016年10月
Detection of Francisella tularensis and analysis of bacterial growth in ticks in Japan
LETTERS IN APPLIED MICROBIOLOGY
- 巻
- 63
- 号
- 4
- 開始ページ
- 240
- 終了ページ
- 246
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1111/lam.12616
- 出版者・発行元
- WILEY-BLACKWELL
Francisella tularensis is distributed in the Northern hemisphere and it is the bacterial agent responsible for tularaemia, a zoonotic disease. We collected 4 527 samples of DNA from ticks in Japan, which were then analysed by real-time PCR and nested PCR. Francisella DNA was detected by real-time PCR in 2.15% (45/2 093) of Ixodes ovatus, 0.66% (14/2 107) of I. persulcatus, 8.22% (6/73) of I. monospinosus and 0.72% (1/138) of Haemaphysalis flava specimens. Finally, Francisella DNA was detected by nested PCR in 42 and five samples I. ovatus and I. persulcatus, respectively, which were positive according to real-time PCR. Phylogenetic analysis showed that the sequence from I. ovatus and I. persulcatus were clustered with F. tularensis type B strains distributed in Eurasia. Microinjected live F. tularensis persisted in ticks, whereas heat-killed F. tularensis decreased. Microinjected F. tularensis hlyD mutant decreased in ticks significantly compared to parent strain, thereby suggesting that HlyD in F. tularensis contributes to the adaptation or survive of bacterial infection in ticks.
- リンク情報
- ID情報
-
- DOI : 10.1111/lam.12616
- ISSN : 0266-8254
- eISSN : 1472-765X
- Web of Science ID : WOS:000386023300002