論文

査読有り 国際誌
2020年8月14日

A Nodal/Eph signalling relay drives the transition from apical constriction to apico-basal shortening in ascidian endoderm invagination.

Development (Cambridge, England)
  • Ulla-Maj Fiuza
  • ,
  • Takefumi Negishi
  • ,
  • Alice Rouan
  • ,
  • Hitoyoshi Yasuo
  • ,
  • Patrick Lemaire

147
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記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1242/dev.186965

Gastrulation is the first major morphogenetic event during animal embryogenesis. Ascidian gastrulation starts with the invagination of 10 endodermal precursor cells between the 64- and late 112-cell stages. This process occurs in the absence of endodermal cell division and in two steps, driven by myosin-dependent contractions of the acto-myosin network. First, endoderm precursors constrict their apex. Second, they shorten apico-basally, while retaining small apical surfaces, thereby causing invagination. The mechanisms that prevent endoderm cell division, trigger the transition between step 1 and step 2, and drive apico-basal shortening have remained elusive. Here, we demonstrate a conserved role for Nodal and Eph signalling during invagination in two distantly related ascidian species, Phallusia mammillata and Ciona intestinalis Specifically, we show that the transition to step 2 is triggered by Nodal relayed by Eph signalling. In addition, our results indicate that Eph signalling lengthens the endodermal cell cycle, independently of Nodal. Finally, we find that both Nodal and Eph signals are dispensable for endoderm fate specification. These results illustrate commonalities as well as differences in the action of Nodal during ascidian and vertebrate gastrulation.

リンク情報
DOI
https://doi.org/10.1242/dev.186965
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/32665244

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