論文

査読有り
2015年12月

Distinct modes of mitotic spindle orientation align cells in the dorsal midline of ascidian embryos

DEVELOPMENTAL BIOLOGY
  • Takefumi Negishi
  • ,
  • Hitoyoshi Yasuo

408
1
開始ページ
66
終了ページ
78
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.ydbio.2015.09.019
出版者・発行元
ACADEMIC PRESS INC ELSEVIER SCIENCE

The orientation of cell division can have important consequences on the choice of cell fates adopted by each daughter cell as well as on the architecture of the tissue within which the dividing cell resides. We have studied in detail the oriented cell divisions that take place in the dorsal midline of the ascidian embryo. The dorsal midline cells of the ascidian embryo emerge following an asymmetric cell division oriented along the animal-vegetal (A-V) axis. This division generates the NN (Notochord-Neural) cell at the margin and the E (Endoderm) cell more vegetally. Deviating from the default mode of cell division, these sister cells divide again along the A-V axis to generate a column of four cells. We describe these cell divisions in detail. We show that the NN cell mitotic spindle rotates 90 degrees to align along the A-V axis while the E cell spindle forms directly along the axis following the asymmetric migration of its centrosomes. We combine live imaging, embryo manipulations and pharmacological modulation of cytoskeletal elements to address the mechanisms underlying these distinct subcellular behaviours. Our evidence suggests that, in E cells, aster asymmetry together with the E cell shape contribute to the asymmetric centrosome migration. In NN cells, an intrinsic cytoplasmic polarisation of the cell results in the accumulation of dynein to the animal pole side. Our data support a model in which a dynein-dependent directional cytoplasmic pulling force may be responsible for the NN cell spindle rotation. (C) 2015 Elsevier Inc. All rights reserved.

Web of Science ® 被引用回数 : 11

リンク情報
DOI
https://doi.org/10.1016/j.ydbio.2015.09.019
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/26452428
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000366956700009&DestApp=WOS_CPL

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