2013年8月
In Vivo Fluorescent Adenosine 5'-Triphosphate (ATP) Imaging of Drosophila melanogaster and Caenorhabditis elegans by Using a Genetically Encoded Fluorescent ATP Biosensor Optimized for Low Temperatures
ANALYTICAL CHEMISTRY
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- 巻
- 85
- 号
- 16
- 開始ページ
- 7889
- 終了ページ
- 7896
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1021/ac4015325
- 出版者・発行元
- AMER CHEMICAL SOC
Adenosine 5'-triphosphate (ATP) is the major energy currency of all living organisms. Despite its important 4 functions, the spatiotemporal dynamics of ATP levels inside 3 living multicellular organisms is unclear. In this study, we 2 modified the genetically encoded Forster resonance energy transfer (FRET)-based ATP biosensor ATeam to optimize its affinity at low temperatures. This new biosensor, AT1.03NL, detected ATP changes inside Drosophila S2 cells more sensitively than the original biosensor did, at 25 degrees C. By expressing AT1.03NL in Drosophila melanogaster and Caenorhabditis elegans, we succeeded in imaging the in vivo ATP dynamics of these model animals at single-cell resolution.
- リンク情報
- ID情報
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- DOI : 10.1021/ac4015325
- ISSN : 0003-2700
- PubMed ID : 23875533
- Web of Science ID : WOS:000323471800038