論文

査読有り
2011年7月

Intracellular colocalization of HAP1/STBs with steroid hormone receptors and its enhancement by a proteasome inhibitor

EXPERIMENTAL CELL RESEARCH
  • Ryutaro Fujinaga
  • ,
  • Yukio Takeshita
  • ,
  • Kazuhiro Yoshioka
  • ,
  • Hiroyuki Nakamura
  • ,
  • Shuhei Shinoda
  • ,
  • Md Nabiul Islam
  • ,
  • Mir Rubayet Jahan
  • ,
  • Akie Yanai
  • ,
  • Keiji Kokubu
  • ,
  • Koh Shinoda

317
12
開始ページ
1689
終了ページ
1700
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.yexcr.2011.05.004
出版者・発行元
ELSEVIER INC

The stigmoid body (STB) is a cytoplasmic inclusion containing huntingtin-associated protein 1 (HAP1), and HAP1/STB formation is induced by transfection of the HAP1 gene into cultured cells. In the present study, we examined the intracellular colocalization of HAP1/STB5 with steroid hormone receptors (SHRs), including the androgen receptor (AR), estrogen receptor, glucocorticoid receptor (GR), and mineralocorticoid receptor, in COS-7 cells cotransfected with HAP1 and each receptor. We found that C-terminal ligand-binding domains of all SHRs had potential for colocalization with HAP1/STB5, whereas only AR and GR were clearly colocalized with HAP1/STBs when each full-length SHR was coexpressed with HAP1. In addition, it appeared that HAP1/STB5 did not disrupt GR and AR functions because the receptors on HAP1/STBs maintained nuclear translocation activity in response to their specific ligands. When the cells were treated with a proteasome inhibitor, GR and AR localized outside HAP1/STB5 translocated into the nucleus, whereas the receptors colocalized with HAP1/STB5 persisted in their colocalization even after treatment with their ligands. Therefore, HAP1/STB5 may be involved in cytoplasmic modifications of the nuclear translocation of GR and AR in a ubiquitin-proteasome system. (c) 2011 Elsevier Inc. All rights reserved.

Web of Science ® 被引用回数 : 6

リンク情報
DOI
https://doi.org/10.1016/j.yexcr.2011.05.004
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/21609716
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000292181600005&DestApp=WOS_CPL

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