論文

2015年9月

Physical interaction of CCN2 with diverse growth factors involved in chondrocyte differentiation during endochondral ossification

JOURNAL OF CELL COMMUNICATION AND SIGNALING
  • Hany Mohamed Khattab
  • ,
  • Eriko Aoyama
  • ,
  • Satoshi Kubota
  • ,
  • Masaharu Takigawa

9
3
開始ページ
247
終了ページ
254
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s12079-015-0290-x
出版者・発行元
SPRINGER

CCN family member 2 (CCN2) has been shown to promote the proliferation and differentiation of chondrocytes, osteoblasts, osteoclasts, and vascular endothelial cells. In addition, a number of growth factors and cytokines are known to work in harmony to promote the process of chondrogenesis and chondrocyte differentiation toward endochondral ossification. Earlier we showed that CCN2 physically interacts with some of them, suggesting that multiple effects of CCN2 on various differentiation stages of chondrocytes may be attributed to its interaction with these growth factors and cytokines. However, little is known about the functional interaction occurring between CCN2 and other growth factors and cytokines in promoting chondrocyte proliferation and differentiation. In this study we sought to shed light on the binding affinities between CCN2 and other essential growth factors and cytokines known to be regulators of chondrocyte differentiation. Using the surface plasmon resonance assay, we analyzed the dissociation constant between CCN2 and each of the following: TGF-beta 1, TGF-beta 3, IGF-I, IGF-II, PDGF-BB, GDF5, PTHrP, and VEGF. We found a strong association between CCN2 and VEGF, as well as a relatively high association with TGF-beta 1, TGF-beta 3, PDGF-BB, and GDF-5. However, the sensorgrams obtained for possible interaction between CCN2 and IGF-I, IGF-II or PTHrP showed no response. This study underlines the correlation between CCN2 and certain other growth factors and cytokines and suggests the possible participation of such interaction in the process of chondrogenesis and chondrocyte differentiation toward endochondral ossification.

リンク情報
DOI
https://doi.org/10.1007/s12079-015-0290-x
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000363377200005&DestApp=WOS_CPL
ID情報
  • DOI : 10.1007/s12079-015-0290-x
  • ISSN : 1873-9601
  • eISSN : 1873-961X
  • Web of Science ID : WOS:000363377200005

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