論文

査読有り 最終著者
2020年9月

Comparison of the Hybrid Capture II Method with a PCR-Based Screening Method Using a Carboxyfluorescein-Labeled Primer for Detecting Human Papillomavirus in Cervicovaginal Liquid-Based Cytology

Journal of Molecular Pathology
  • Yusuke Saiki
  • ,
  • Yuka Gion
  • ,
  • Asami Nishikori
  • ,
  • Yoshiaki Norimatsu
  • ,
  • Yasuharu Sato

1
1
開始ページ
9
終了ページ
18
記述言語
掲載種別
研究論文(学術雑誌)
DOI
10.3390/jmp1010003
出版者・発行元
MDPI AG

Objective: Human papillomaviruses (HPVs) are DNA viruses, of which over 120 types have been identified. The main screening methods for HPV-DNA include the hybrid capture II (HC-II) and polymerase chain reaction (PCR) assays. Liquid-based cytology (LBC) is a high-quality technique developed to improve the diagnostic reliability of traditional Papanicolaou tests (Pap tests). However, relatively few studies have compared the efficacy of PCR and HC-II assays using cervicovaginal LBC specimens. In this study, we conducted a comparative analysis with results derived from the HC-II assay to assess whether a PCR-based assay using a novel carboxyfluorescein (FAM)-labeled primer could be applied to cervicovaginal LBC specimens. Methods and Results: We analyzed 59 specimens diagnosed as atypical squamous cells of undetermined significance (ASCUS) by Pap tests. After extracting DNA from cervicovaginal LBC specimens, we performed PCR using a FAM-labeled consensus primer, and then conducted fragment analysis to confirm the results. The value of the kappa statistic measuring the agreement between the PCR and HC-II results was 0.8557, or “almost perfect agreement.” Conclusion: Our novel HPV-PCR assay can be successfully applied to cervicovaginal LBC specimens for the detection of HPV subtypes.

リンク情報
DOI
https://doi.org/10.3390/jmp1010003
URL
https://www.mdpi.com/2673-5261/1/1/3
ID情報
  • DOI : 10.3390/jmp1010003
  • eISSN : 2673-5261
  • ORCIDのPut Code : 80336801

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