Papers

Feb 1, 2018

Cholesterol-α-glucosyltransferase gene is present in most Helicobacter species including gastric non-Helicobacter pylori helicobacters obtained from Japanese patients

Helicobacter
  • Masatomo Kawakubo
  • ,
  • Kazuki Horiuchi
  • ,
  • Takehisa Matsumoto
  • ,
  • Jun Nakayama
  • ,
  • Taiji Akamatsu
  • ,
  • Tsutomu Katsuyama
  • ,
  • Hiroyoshi Ota
  • ,
  • Junji Sagara

Volume
23
Number
1
First page
-
Last page
Language
English
Publishing type
Research paper (scientific journal)
DOI
10.1111/hel.12449
Publisher
Blackwell Publishing Ltd

Background: Non-Helicobacter pylori helicobacters (NHPHs) besides H. pylori infect human stomachs and cause chronic gastritis and mucosa-associated lymphoid tissue lymphoma. Cholesteryl-α-glucosides have been identified as unique glycolipids present in H. pylori and some Helicobacter species. Cholesterol-α-glucosyltransferase (αCgT), a key enzyme for the biosynthesis of cholesteryl-α-glucosides, plays crucial roles in the pathogenicity of H. pylori. Therefore, it is important to examine αCgTs of NHPHs. Materials and Methods: Six gastric NHPHs were isolated from Japanese patients and maintained in mouse stomachs. The αCgT genes were amplified by PCR and inverse PCR. We retrieved the αCgT genes of other Helicobacter species by BLAST searches in GenBank. Results: αCgT genes were present in most Helicobacter species and in all Japanese isolates examined. However, we could find no candidate gene for αCgT in the whole genome of Helicobacter cinaedi and several enterohepatic species. Phylogenic analysis demonstrated that the αCgT genes of all Japanese isolates show high similarities to that of a zoonotic group of gastric NHPHs including Helicobacter suis, Helicobacter heilmannii, and Helicobacter ailurogastricus. Of 6 Japanese isolates, the αCgT genes of 4 isolates were identical to that of H. suis, and that of another 2 isolates were similar to that of H. heilmannii and H. ailurogastricus. Conclusions: All gastric NHPHs examined showed presence of αCgT genes, indicating that αCgT may be beneficial for these helicobacters to infect human and possibly animal stomachs. Our study indicated that NHPHs could be classified into 2 groups, NHPHs with αCgT genes and NHPHs without αCgT genes.

Link information
DOI
https://doi.org/10.1111/hel.12449
ID information
  • DOI : 10.1111/hel.12449
  • ISSN : 1523-5378
  • ISSN : 1083-4389
  • SCOPUS ID : 85040245337

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