論文

査読有り
2017年10月

Cloning of Helicobacter suis cholesterol alpha-glucosyltransferase and production of an antibody capable of detecting it in formalin-fixed, paraffin-embedded gastric tissue sections

HISTOCHEMISTRY AND CELL BIOLOGY
  • Masatomo Kawakubo
  • ,
  • Kazuki Horiuchi
  • ,
  • Hitomi Komura
  • ,
  • Yoshiko Sato
  • ,
  • Masayoshi Kato
  • ,
  • Meguru Ikeyama
  • ,
  • Mana Fukushima
  • ,
  • Shigenori Yamada
  • ,
  • Satoshi Ishizone
  • ,
  • Takehisa Matsumoto
  • ,
  • Hiroyoshi Ota
  • ,
  • Junji Sagara
  • ,
  • Jun Nakayama

148
4
開始ページ
463
終了ページ
471
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s00418-017-1582-4
出版者・発行元
SPRINGER

Helicobacter suis (H. suis), formerly called Helicobacter heilmannii type 1 (H. heilmannii), is a gram-negative bacterium of the Helicobacter species. This pathogen infects the stomach of humans and animals such as dogs, cats, pigs, and rodents, the latter giving rise to zoonotic infection. Here, we generated a H. suis-specific antibody useful for immunohistochemistry with formalin-fixed, paraffin-embedded tissue sections. To do so, we began by cloning the gene encoding H. suis cholesterol alpha-glucosyltransferase (alpha CgT). alpha CgT is the key enzyme responsible for biosynthesis of cholesteryl alpha-d-glucopyranoside (CGL), a major cell wall component of Helicobacter species including H. suis. The deduced amino acid sequence of H. suis alpha CgT had 56% identity with the corresponding Helicobacter pylori (H. pylori). We then developed a polyclonal antibody (anti-Hh-I205R) by immunizing rabbits with a 205 amino acid H. suis alpha CgT fragment. Immunohistochemistry with the anti-Hh-I205R antibody could differentiate H. suis from H. pylori in gastric mucosa sections derived from mice infected with either pathogen. We then probed formalin-fixed, paraffin-embedded sections of human gastric mucosa positive for H. suis infection with the anti-Hh-I205R antibody and detected positive staining. These results indicate that anti-Hh-I205R antibody is specific for H. suis alpha CgT and useful to detect H. suis in gastric specimens routinely analyzed in pathological examinations.

Web of Science ® 被引用回数 : 2

リンク情報
DOI
https://doi.org/10.1007/s00418-017-1582-4
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000410786600011&DestApp=WOS_CPL

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