2018年11月29日
Nanodiscs for Structural Biology in a Membranous Environment
Chemical and Pharmaceutical Bulletin
- ,
- ,
- 巻
- 67
- 号
- 4
- 開始ページ
- 321
- 終了ページ
- 326
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1248/cpb.c18-00941
- 出版者・発行元
- Pharmaceutical Society of Japan
© 2019 The Pharmaceutical Society of Japan The structures of many membrane proteins have been analyzed in detergent micelles. However, the environment of detergent micelles differs somewhat from that of the lipid bilayer, where membrane proteins exhibit physiological functions. Therefore, a more membrane-like environment has been awaited for structural analysis of membrane proteins. Nanodiscs are “hockey-puck”-shaped lipid bilayer particles that distribute in a monodispersed manner in aqueous solution. We review how nanodiscs or protein-reconstituted nanodiscs are prepared and how they are utilized to analyze protein structure, dynamics, and interactions with lipid molecules using solution NMR and cryo-electron microscopy.
- リンク情報
- ID情報
-
- DOI : 10.1248/cpb.c18-00941
- ISSN : 0009-2363