MISC

2016年6月

HMGB1 Translocation After Ischemia in the Ovine Fetal Brain

JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY
  • Jiyong Zhang
  • ,
  • Daniel Klufas
  • ,
  • Karina Manalo
  • ,
  • Kwame Adjepong
  • ,
  • Joanne O. Davidson
  • ,
  • Guido Wassink
  • ,
  • Laura Bennet
  • ,
  • Alistair J. Gunn
  • ,
  • Edward G. Stopa
  • ,
  • Keyue Liu
  • ,
  • Masahiro Nishibori
  • ,
  • Barbara S. Stonestreet

75
6
開始ページ
527
終了ページ
538
記述言語
英語
掲載種別
DOI
10.1093/jnen/nlw030
出版者・発行元
OXFORD UNIV PRESS INC

Inflammation contributes to the evolution of hypoxic-ischemic (HI) brain injury. High-mobility group box-1 (HMGB1) is a nuclear protein that is translocated from the nucleus and released after ischemia in adult rodents and thereby initiates inflammatory responses. However, there is very little information regarding the effects of HI on HMGB1 in immature brains. To investigate the effects of HI on HMGB1 in the term-equivalent fetal brain, ovine fetuses at 127 days gestation were studied after 30 minutes of carotid occlusion. Groups were sham-control and ischemia with 48 hours and ischemia with 72 hours of reperfusion. By immunohistochemistry, HMGB1 was found to be localized primarily in cell nuclei and partially in cytoplasmic compartments in the cerebral cortex of controls. Ischemia increased the area fraction of neuronal cells with cytoplasmic HMGB1 staining, and Western immunoblot revealed that cytosolic HMGB1 expression increased after ischemia (p < 0.05) and decreased in nuclei in ischemic versus the sham-control brains (p < 0.05). These data indicate that HMGB1 translocates from the nuclear to cytosolic compartments after ischemic brain injury in fetal sheep. This translocation may enable the action of HMGB1 as a proinflammatory cytokine that contributes to HI injury in the developing brain.

Web of Science ® 被引用回数 : 11

リンク情報
DOI
https://doi.org/10.1093/jnen/nlw030
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000377665000005&DestApp=WOS_CPL

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