論文

査読有り 国際誌
2019年12月2日

VISTA Is Crucial for Corneal Allograft Survival and Maintenance of Immune Privilege.

Investigative ophthalmology & visual science
  • Tomoyuki Kunishige
  • ,
  • Hiroko Taniguchi
  • ,
  • Tatsukuni Ohno
  • ,
  • Miyuki Azuma
  • ,
  • Junko Hori

60
15
開始ページ
4958
終了ページ
4965
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1167/iovs.19-27322

Purpose: V-domain immunoglobulin suppressor of T cell activation (VISTA) is a novel immune checkpoint receptor and ligand for regulating T cell proliferation and cytokine production. The purpose of the present study was to determine the role of VISTA in the immune privilege of corneal allografts. Methods: Expression of VISTA mRNA in mouse eyes was assessed with reverse-transcription PCR. Corneas of C57BL/6 mice were orthotopically transplanted into the eyes of BALB/c wild-type recipients treated with anti-VISTA mAb, and graft survival was assessed. A separate set of BALB/c mice treated with anti-VISTA mAb or rat IgG received injection of C57BL/6 splenocytes into the anterior chamber, and induction of allospecific anterior chamber-associated immune deviation (ACAID) was assessed. CD4+ and CD8+ T cells in the spleen were assessed with flow cytometry. Results: VISTA mRNA was constitutively expressed in the cornea, and the expression of VISTA was localized to CD11b+ cells on the corneal stroma. Survival of allografts treated with anti-VISTA mAb was less than that of the control. ACAID was induced less efficiently in BALB/c mice treated with VISTA mAb. The proportions of CD8+ T cells and CD8+ CD103+ T cells (CD8+ T regulatory cells) in the spleen of BALB/c mice treated with anti-VISTA mAb were significantly lower than those of the control. Conclusions: VISTA may play an essential role in the acceptance of corneal allografts via involvement with allospecific ACAID, which suppresses T cell infiltration into the cornea.

リンク情報
DOI
https://doi.org/10.1167/iovs.19-27322
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/31790558

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