論文

査読有り
2019年2月

Aggregation makes a protein allergenic at the challenge phase of basophil-mediated allergy in mice.

Int. Immunol.
  • Toshihisa Nagao
  • ,
  • Yoshinori Yamanishi
  • ,
  • Kensuke Miyake
  • ,
  • Mio Teranishi
  • ,
  • Saori Takahashi
  • ,
  • Soichiro Yoshikawa
  • ,
  • Yohei Kawano
  • ,
  • Hajime Karasuyama

31
1
開始ページ
41
終了ページ
49
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1093/intimm/dxy062

A hapten is a small molecule that is not immunogenic on its own but can stimulate the production of antibodies at the sensitization phase when conjugated to carrier proteins. The hapten then reacts specifically with the antibodies generated against it to elicit an immune or allergic response at the challenge phase. Here, we compared various carrier proteins conjugated with the same hapten in their ability to induce hapten-specific IgE-mediated allergic responses in vitro and in vivo, and characterized the nature of carrier proteins that determines the magnitude of response at the challenge phase of allergic reactions. Hapten 2,4,6-trinitrophenol (TNP)-conjugated ovalbumin (TNP-OVA) and bovine serum albumin (TNP-BSA) elicited TNP-specific, mast cell-dependent, immediate-type allergic reactions at a comparable level in mice that had been passively sensitized with TNP-specific IgE. In contrast, TNP-OVA but not TNP-BSA efficiently induced a basophil-dependent, IgE-mediated chronic allergic inflammation (IgE-CAI), even though both proteins could stimulate basophils in vitro at a comparable level. By comparing different carrier proteins and structurally modifying them, we found that the

リンク情報
DOI
https://doi.org/10.1093/intimm/dxy062
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/30239735