論文

査読有り
2018年3月27日

High-sensitivity virus and mycoplasma screening test reveals high prevalence of parvovirus B19 infection in human synovial tissues and bone marrow

Stem Cell Research and Therapy
  • Ken Watanabe
  • ,
  • Koji Otabe
  • ,
  • Norio Shimizu
  • ,
  • Keiichirou Komori
  • ,
  • Mitsuru Mizuno
  • ,
  • Hisako Katano
  • ,
  • Hideyuki Koga
  • ,
  • Ichiro Sekiya

9
1
開始ページ
80
終了ページ
80
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1186/s13287-018-0811-7
出版者・発行元
BioMed Central Ltd.

Background: Latent microorganism infection is a safety concern for the clinical application of mesenchymal stem cells (MSCs). The aim of this study is to investigate the frequencies and sensitivities of the latent virus and mycoplasma infections in synovium, bone marrow, peripheral blood cells, and blood plasma and cultured synovial MSCs. Methods: Total DNA and RNA of the synovium (n = 124), bone marrow (n = 123), peripheral blood cells (n = 121), plasma (n = 121), and 14-day cultured synovial MSCs (n = 63) were collected from patients who underwent total knee arthroplasty or anterior ligament reconstruction after written informed consents were obtained. The multiplex polymerase chain reaction (PCR) primers were designed to quantitatively measure the representative genomes of 13 DNA viruses, 6 RNA viruses, and 9 mycoplasmas. Multi-spliced mRNA detection and virus spike test were also performed to demonstrate the sensitivity of synovial MSCs to the candidate pathogens. Results: In synovium and bone marrow, the positive rates of parvovirus B19 genome were significantly higher than in peripheral blood cells (18.7% and 22% vs. 0.8%, respectively). Multi-alignment analysis of amplified and sequenced viral target genes showed the proximity of the parvovirus B19 gene from different tissue in the same patients. Synovial MSCs cultured for 14 days were positive for virus infection only in two patients (2/62 = 3%). Parvovirus B19 multi-spliced mRNAs were not detected in these two samples. Virus spike test demonstrated the sensitivity of synovial MSCs to herpes simplex virus (HSV)1 and cytomegalovirus (CMV), but not to parvovirus B19. Conclusion: This study revealed a relatively high incidence of latent parvovirus B19 in synovium and bone marrow tissue.

リンク情報
DOI
https://doi.org/10.1186/s13287-018-0811-7
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/29587847

エクスポート
BibTeX RIS