論文

査読有り
2017年6月

Complete human serum maintains viability and chondrogenic potential of human synovial stem cells: suitable conditions for transplantation

STEM CELL RESEARCH & THERAPY
  • Mitsuru Mizuno
  • ,
  • Hisako Katano
  • ,
  • Koji Otabe
  • ,
  • Keiichiro Komori
  • ,
  • Yuji Kohno
  • ,
  • Shizuka Fujii
  • ,
  • Nobutake Ozeki
  • ,
  • Masafumi Horie
  • ,
  • Kunikazu Tsuji
  • ,
  • Hideyuki Koga
  • ,
  • Takeshi Muneta
  • ,
  • Ichiro Sekiya

8
1
開始ページ
144
終了ページ
144
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1186/s13287-017-0596-0
出版者・発行元
BIOMED CENTRAL LTD

Background: In our clinical practice, we perform transplantations of autologous synovial mesenchymal stem cells (MSCs) for cartilage and meniscus regenerative medicine. One of the most important issues to ensuring clinical efficacy involves the transport of synovial MSCs from the processing facility to the clinic. Complete human serum (100% human serum) is an attractive candidate material in which to suspend synovial MSCs for their preservation during transport. The purpose of this study was to investigate whether complete human serum maintained MSC viability and chondrogenic potential and to examine the optimal temperature conditions for the preservation of human synovial MSCs.
Methods: Human synovium was harvested from the knees of 14 donors with osteoarthritis during total knee arthroplasty. Passage 2 synovial MSCs were suspended at 2 million cells/100 mu L in Ringer's solution or complete human serum at 4, 13, and 37 degrees C for 48 h. These cells were analyzed for live cell rates, cell surface marker expression, metabolic activity, proliferation, and adipogenic, calcification, and chondrogenic differentiation potentials before and after preservation.
Results: After preservation, synovial MSCs maintained higher live cell rates in human serum than in Ringer's solution at 4 and 13 degrees C. Synovial MSCs preserved in human serum at 4 and 13 degrees C also maintained high ratios of propidium iodide(-) and annexin V- cells. MSC surface marker expression was not altered in cells preserved at 4 and 13 degrees C. The metabolic activities of cells preserved in human serum at 4 and 13 degrees C was maintained, while significantly reduced in other conditions. Replated MSCs retained their proliferation ability when preserved in human serum at 4 and 13 degrees C. Adipogenesis and calcification potential could be observed in cells preserved in each condition, whereas chondrogenic potential was retained only in cells preserved in human serum at 4 and 13 degrees C.
Conclusion: The viability and chondrogenic potential of synovial MSCs were maintained when the cells were suspended in human serum at 4 and 13 degrees C.

Web of Science ® 被引用回数 : 3

リンク情報
DOI
https://doi.org/10.1186/s13287-017-0596-0
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/28610596
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000403638500002&DestApp=WOS_CPL

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