論文

査読有り
2017年5月

Yields and chondrogenic potential of primary synovial mesenchymal stem cells are comparable between rheumatoid arthritis and osteoarthritis patients

STEM CELL RESEARCH & THERAPY
  • Yuji Kohno
  • ,
  • Mitsuru Mizuno
  • ,
  • Nobutake Ozeki
  • ,
  • Hisako Katano
  • ,
  • Keiichiro Komori
  • ,
  • Shizuka Fujii
  • ,
  • Koji Otabe
  • ,
  • Masafumi Horie
  • ,
  • Hideyuki Koga
  • ,
  • Kunikazu Tsuji
  • ,
  • Mikio Matsumoto
  • ,
  • Haruka Kaneko
  • ,
  • Yuji Takazawa
  • ,
  • Takeshi Muneta
  • ,
  • Ichiro Sekiya

8
1
開始ページ
115
終了ページ
115
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1186/s13287-017-0572-8
出版者・発行元
BIOMED CENTRAL LTD

Background: Mesenchymal stem cells derived from the synovial membrane (synovial MSCs) are a candidate cell source for regenerative medicine of cartilage and menisci due to their high chondrogenic ability. Regenerative medicine can be expected for RA patients with the inflammation well-controlled as well as OA patients and transplantation of synovial MSCs would also be a possible therapeutic treatment. Some properties of synovial MSCs vary dependent on the diseases patients have, and whether or not the pathological condition of RA affects the chondrogenesis of synovial MSCs remains controversial. The purpose of this study was to compare the properties of primary synovial MSCs between RA and OA patients.
Methods: Human synovial tissue was harvested during total knee arthroplasty from the knee joints of eight patients with RA and OA respectively. Synovial nucleated cells were cultured for 14 days. Total cell yields, surface markers, and differentiation potentials were analyzed for primary synovial MSCs.
Results: Nucleated cell number per 1 mg synovium was 8.4 +/- 3.9 thousand in RA and 8.0 +/- 0.9 thousand in OA. Total cell number after 14-day culture/1 mg synovium was 0.7 +/- 0.4 million in RA and 0.5 +/- 0.3 million in OA, showing no significant difference between in RA and OA. Cells after 14-day culture were mostly positive for CD44, CD73, CD90, CD105, negative for CD45 both in RA and OA. There was no significant difference for the cartilage pellet weight and sGAG content per pellet between in RA and OA. Both oil red O-positive colony rate and alizarin red-positive colony rate were similar in RA and OA.
Conclusions: Yields, surface markers and chondrogenic potential of primary synovial MSCs in RA were comparable to those in OA. Synovium derived from RA patients can be the cell source of MSCs for cartilage and meniscus regeneration.

Web of Science ® 被引用回数 : 14

リンク情報
DOI
https://doi.org/10.1186/s13287-017-0572-8
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/28511664
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000401481700002&DestApp=WOS_CPL

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