論文

査読有り
2017年5月

TNFα promotes proliferation of human synovial MSCs while maintaining chondrogenic potential.

PLoS ONE
  • Mikio Shioda
  • ,
  • Takeshi Muneta
  • ,
  • Kunikazu Tsuji
  • ,
  • Mitsuru Mizuno
  • ,
  • Keiichiro Komori
  • ,
  • Hideyuki Koga
  • ,
  • Ichiro Sekiya

12
5
開始ページ
e0177771
終了ページ
e0177771
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1371/journal.pone.0177771

Synovial mesenchymal stem cells (MSCs) are a candidate cell source for cartilage and meniscus regeneration. If we can proliferate synovial MSCs more effectively, we can expand clinical applications to patients with large cartilage and meniscus lesions. TNFα is a pleiotropic cytokine that can affect the growth and differentiation of cells in the body. The purpose of this study was to examine the effect of TNFα on proliferation, chondrogenesis, and other properties of human synovial MSCs. Passage 1 human synovial MSCs from 2 donors were cultured with 2.5 x 10-12~10-7 g/ml, 10 fold dilution series of TNFα for 14 days, then the cell number and colony number was counted. The effect of the optimum dose of TNFα on proliferation was also examined in synovial MSCs from 6 donors. Chondrogenic potential of synovial MSCs pretreated with TNFα was evaluated in 6 donors. The expressions of 12 surface antigens were also examined in 3 donors.2.5 ng/ml and higher concentration of TNFα significantly increased cell number/dish and cell number/colony in both donors. The effect of 25 ng/ml TNFα was confirmed in all 6 donors. There was no significant difference in the weight, or amount of glycosam

Web of Science ® 被引用回数 : 8

リンク情報
DOI
https://doi.org/10.1371/journal.pone.0177771
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/28542363
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000401672400081&DestApp=WOS_CPL

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