Papers

Peer-reviewed
Jul 1, 2022

Diagnostic value of recombinant nanoluciferase fused Toxoplasma gondii antigens in Luciferase-linked Antibody Capture Assay (LACA) for Toxoplasma infection in pigs.

The Journal of veterinary medical science
  • Hieu Duc Duong
  • ,
  • Yuji Taniguchi
  • ,
  • Yasuhiro Takashima
  • ,
  • Satoshi Sekiguchi
  • ,
  • Khin Myo Aye
  • ,
  • Parnian Ahmadi
  • ,
  • Linh Khanh Bui
  • ,
  • Takao Irie
  • ,
  • Eiji Nagayasu
  • ,
  • Ayako Yoshida

Volume
84
Number
7
First page
905
Last page
913
Language
English
Publishing type
Research paper (scientific journal)
DOI
10.1292/jvms.22-0099

Toxoplasmosis is a widespread protozoan zoonosis. Since ingesting undercooked meat harboring Toxoplasma gondii cyst is considered one of the major transmission routes to humans, the screening of T. gondii in meat-producing animals can reduce the risk of food-borne toxoplasmosis in humans. Among serological diagnostic methods, Luciferase-linked Antibody Capture Assay (LACA) has been found to be a promising platform with high sensitivity and specificity. In this study, we aimed to evaluate recombinant nanoluciferase fused-T. gondii antigens (rNluc-GRA6, rNluc-GRA7, rNluc-GRA8 and rNluc-BAG1) for their potential use in LACA for pigs. As a result, the sensitivity of GRA6-, GRA7-, GRA8- and BAG1-LACA were 70.0%, 80.0%, 80.0% and 30.0% with specificity 87.0%, 81.5%, 74.1% and 50.0%, respectively. The cocktail LACA using a mixture of rNluc-GRA6, rNluc-GRA7 and rNluc-GRA8 indicated higher sensitivity (90.0%) and a similar specificity (96.3%) in comparison with the commercial ELISA kit. Compared to the Dye-Test as a reference test, cocktail LACA showed strong agreement (kappa value=0.811) when we assessed pig sera collected at the slaughterhouse. In addition, we also successfully established the rapid LACA format for the detection of Toxoplasma infection in pigs (called Rapid-LACA) in which the test could be performed within 30 min. In Rapid-LACA, the protein A pre-coated/blocked plates could be preserved at -30°C, 4°C or room temperature conditions for at least two months without compromising on the quality of assay.

Link information
DOI
https://doi.org/10.1292/jvms.22-0099
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/35584943
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9353080
ID information
  • DOI : 10.1292/jvms.22-0099
  • Pubmed ID : 35584943
  • Pubmed Central ID : PMC9353080

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