論文

2005年7月

Translational repression by the cis-acting element of structure-anchored repression (CAESAR) of human ctgf/ccn2 mRNA

FEBS LETTERS
  • S Kubota
  • ,
  • Y Mukudai
  • ,
  • NH Moritani
  • ,
  • K Nakao
  • ,
  • K Kawata
  • ,
  • M Takigawa

579
17
開始ページ
3751
終了ページ
3758
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.febslet.2005.05.068
出版者・発行元
ELSEVIER SCIENCE BV

The cis-acting element of structure-anchored repression (CAESAR) is a post-transcriptional regulatory element of gene expression, which is located in the 3'-untranslated region (UTR) of the human ccn2 gene (ctgflccn2). In this report, the repression mechanism of CAESAR, as well as the structural requirement, was investigated. Removal of minor stem-loops from CAESAR resulted in proportional attenuation of the repressive function, whereas removal of the single bulge or modification of primary nucleotide sequence did not affect its functionality. In light of functional mechanism, CAESAR exerted no significant effects on stability or nuclear export of the cis-linked mRNA. However, this element significantly interfered with the association of such mRNA on ribosome and slowed down the translation process thereafter in vitro. A translation repression mechanism by RNA secondary structure to determine the basal ctgflccn2 expression level was uncovered herein. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Web of Science ® 被引用回数 : 17

リンク情報
DOI
https://doi.org/10.1016/j.febslet.2005.05.068
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000230335600045&DestApp=WOS_CPL

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