論文

2008年9月

Distribution, gene expression, and functional role of EphA4 during ossification

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
  • Chisa Kuroda
  • ,
  • Satoshi Kubota
  • ,
  • Kazumi Kawata
  • ,
  • Eriko Aoyama
  • ,
  • Kumi Sumiyoshi
  • ,
  • Morihiko Oka
  • ,
  • Miho Inoue
  • ,
  • Shogo Minagi
  • ,
  • Masaharu Takigawa

374
1
開始ページ
22
終了ページ
27
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.bbrc.2008.06.089
出版者・発行元
ACADEMIC PRESS INC ELSEVIER SCIENCE

EphA4 receptor tyrosine kinase has been shown to be critically involved in neural tissue development. Here, we found EphA4 was also distributed among hypertrophic chondrocytes and osteoblasts in the growth plate of developing mouse long bones. In vitro evaluation revealed that ephA4 expression was elevated Upon hypertrophic differentiation of chondrocytes and that markedly stronger expression was observed in osteoblastic SaOS-2 than chondrocytic HCS-2/8 cells. Of note, RNAi-mediated silencing of ephA4 in SaOS-2 cells resulted in the repression of osteocalcin gene expression and alkaline phosphatase activity. Interestingly, confocal laser-scanning Microscopic analysis revealed the presence of EphA4 molecules in the nucleus as well as on the surface of SaOS-2 cells. These findings are the first indication of a critical role of EphA4 in ossification, especially at the final stage in which osteoblasts and hypertrophic chondrocytes play major roles. (C) 2008 Elsevier Inc. All rights reserved.

Web of Science ® 被引用回数 : 14

リンク情報
DOI
https://doi.org/10.1016/j.bbrc.2008.06.089
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000258798700005&DestApp=WOS_CPL

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