論文

査読有り
2008年6月

Promotion of bone regeneration by CCN2 incorporated into gelatin hydrogel

TISSUE ENGINEERING PART A
  • Takeshi Kikuchi
  • ,
  • Satoshi Kubota
  • ,
  • Koji Asaumi
  • ,
  • Harumi Kawaki
  • ,
  • Takashi Nishida
  • ,
  • Kazumi Kawata
  • ,
  • Shigeru Mitani
  • ,
  • Yasuhiko Tabata
  • ,
  • Toshifumi Ozaki
  • ,
  • Masaharu Takigawa

14
6
開始ページ
1089
終了ページ
1098
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1089/ten.tea.2007.0167
出版者・発行元
MARY ANN LIEBERT, INC

CCN family protein 2/connective tissue growth factor (CCN2/CTGF) is a unique molecule that promotes the entire endochondral ossification process and regeneration of damaged articular cartilage. Also, CCN2 has been shown to enhance the adhesion and migration of bone marrow stromal cells as well as the growth and differentiation of osteoblasts; hence, its utility in bone regeneration has been suggested. Here, we evaluated the effect of CCN2 on the regeneration of an intractable bone defect in a rat model. First, we prepared two recombinant CCN2s of different origins, and the one showing the stronger effect on osteoblasts in vitro was selected for further evaluation, based on the result of an in vitro bioassay. Next, to obtain a sustained effect, the recombinant CCN2 was incorporated into gelatin hydrogel that enabled the gradual release of the factor. Evaluation in vivo indicated that CCN2 continued to be released at least for up to 14 days after its incorporation. Application of the gelatin hydrogel-CCN2 complex, together with a collagen scaffold to the bone defect prepared in a rat femur resulted in remarkable induction of osteoblastic mineralization markers within 2 weeks. Finally, distinct enhancement of bone regeneration was observed 3 weeks after the application of the complex. These results confirm the utility of CCN2 in the regeneration of intractable bone defects in vivo when the factor is incorporated into gelatin hydrogel.

Web of Science ® 被引用回数 : 41

リンク情報
DOI
https://doi.org/10.1089/ten.tea.2007.0167
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/19230129
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000256969800014&DestApp=WOS_CPL

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